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. 1975 Mar;2(3):423–429. doi: 10.1093/nar/2.3.423

Preparation and separation of d(pT)10·n oligonucleotides

AJ Raae 1, JR Lillehaug 1, RK Kleppe 1, K Kleppe 1
PMCID: PMC342847  PMID: 1129141

Abstract

A series of oligomers having the general formula d(pT)10·n, n varying from 2 to 20, has been prepared by enzymatic joining of d(pT)10, annealed on poly dA, employing T4 polynucleotide ligase. The oligomers could be separated on 8 or 12% polyacrylamide gels. Such oligomers may prove useful as molecular weight markers and as initiators for various polymerases.

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Selected References

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  1. Burd J. F., Wells R. D. Synthesis and characterization of the duplex block polymer d(C15A15)-d(T15G15). J Biol Chem. 1974 Nov 25;249(22):7094–7801. [PubMed] [Google Scholar]
  2. Glynn I. M., Chappell J. B. A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity. Biochem J. 1964 Jan;90(1):147–149. doi: 10.1042/bj0900147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gupta N. K., Ohtsuka E., Weber H., Chang S. H., Khorana H. G. Studies on polynucleotides. LXXXVII. The joining of short deoxyribopolynucleotides by DNA-joining enzymes. Proc Natl Acad Sci U S A. 1968 May;60(1):285–292. doi: 10.1073/pnas.60.1.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Lehman I. R., Chien J. R. Persistence of deoxyribonucleic acid polymerase I and its 5'--3' exonuclease activity in PolA mutants of Escherichia coli K12. J Biol Chem. 1973 Nov 25;248(22):7717–7723. [PubMed] [Google Scholar]
  5. Nes I. F., Lillehaug J. R., Kleppe K. DNA polymerase I from Acinetobacter calcoaceticus: enzymatic properties. Eur J Biochem. 1974 Dec 16;50(1):167–175. doi: 10.1111/j.1432-1033.1974.tb03884.x. [DOI] [PubMed] [Google Scholar]
  6. Novogrodsky A., Tal M., Traub A., Hurwitz J. The enzymatic phosphorylation of ribonucleic acid and deoxyribonucleic acid. II. Further properties of the 5'-hydroxyl polynucleotide kinase. J Biol Chem. 1966 Jun 25;241(12):2933–2943. [PubMed] [Google Scholar]
  7. Panet A., van de Sande J. H., Loewen P. C., Khorana H. G., Raae A. J., Lillehaug J. R., Kleppe K. Physical characterization and simultaneous purification of bacteriophage T4 induced polynucleotide kinase, polynucleotide ligase, and deoxyribonucleic acid polymerase. Biochemistry. 1973 Dec 4;12(25):5045–5050. doi: 10.1021/bi00749a003. [DOI] [PubMed] [Google Scholar]
  8. Spiegelman S., Burny A., Das M. R., Keydar J., Schlom J., Trávnícek M., Watson K. Synthetic DNA-RNA hybrids and RNA-RNA duplexes as templates for the polymerases of the oncogenic RNA viruses. Nature. 1970 Oct 31;228(5270):430–432. doi: 10.1038/228430a0. [DOI] [PubMed] [Google Scholar]

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