Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1976 Jan;3(1):1–10. doi: 10.1093/nar/3.1.1

Interactions between 30s ribosomal proteins and 50s subunits of Escherichia coli.

D Jenness, J Bruce, H W Schaup
PMCID: PMC342872  PMID: 765969

Abstract

The interaction between ribosomal proteins of the 30s subunit with intact 50s subunits was investigated. Experiments with mixtures of total 30s proteins indicated that several 30s proteins including protein s4 would form a stable complex with 50s subunits. Further work with pure s4 indicates that this protein binds stoichiometrically to the 50s subunits, probably through protein-nucleic acid interaction. The possible significance of this interaction is discussed.

Full text

PDF
1

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Birge E. A., Kurland C. G. Reversion of a streptomycin-dependent strain of Escherichia coli. Mol Gen Genet. 1970;109(4):356–369. doi: 10.1007/BF00267704. [DOI] [PubMed] [Google Scholar]
  2. Kaltschmidt E., Wittmann H. G. Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins. Anal Biochem. 1970 Aug;36(2):401–412. doi: 10.1016/0003-2697(70)90376-3. [DOI] [PubMed] [Google Scholar]
  3. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  4. Lake J. A., Pendergast M., Kahan L., Nomura M. Localization of Escherichia coli ribosomal proteins S4 and S14 by electron microscopy of antibody-labeled subunits. Proc Natl Acad Sci U S A. 1974 Dec;71(12):4688–4692. doi: 10.1073/pnas.71.12.4688. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lutter L. C., Kurland C. G. Reconstitution of active ribosomes with cross-linked proteins. Nat New Biol. 1973 May 2;243(122):15–17. [PubMed] [Google Scholar]
  6. Morrison C. A., Garrett R. A., Zeichhardt H., Stöffler G. Proteins occurring at, or near, the subunit interface of E. coli ribosomes. Mol Gen Genet. 1973 Dec 31;127(4):359–368. doi: 10.1007/BF00267106. [DOI] [PubMed] [Google Scholar]
  7. Noller H. F., Chang C., Thomas G., Aldridge J. Chemical modification of the transfer RNA and polyuridylic acid binding sites of Escherichia coli 30 s ribosomal subunits. J Mol Biol. 1971 Nov 14;61(3):669–679. doi: 10.1016/0022-2836(71)90071-4. [DOI] [PubMed] [Google Scholar]
  8. Nomura M., Mizushima S., Ozaki M., Traub P., Lowry C. V. Structure and function of ribosomes and their molecular components. Cold Spring Harb Symp Quant Biol. 1969;34:49–61. doi: 10.1101/sqb.1969.034.01.009. [DOI] [PubMed] [Google Scholar]
  9. Oen H., Pellegrini M., Eilat D., Cantor C. R. Identification of 50S proteins at the peptidyl-tRNA binding site of Escherichia coli ribosomes. Proc Natl Acad Sci U S A. 1973 Oct;70(10):2799–2803. doi: 10.1073/pnas.70.10.2799. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ozaki M., Mizushima S., Nomura M. Identification and functional characterization of the protein controlled by the streptomycin-resistant locus in E. coli. Nature. 1969 Apr 26;222(5191):333–339. doi: 10.1038/222333a0. [DOI] [PubMed] [Google Scholar]
  11. Randall-Hazelbauer L. L., Kuland C. G. Identification of three 30S proteins contributing to the ribosomal A site. Mol Gen Genet. 1972;115(3):234–242. doi: 10.1007/BF00268887. [DOI] [PubMed] [Google Scholar]
  12. Schaup H. W., Green M., Kurland C. G. Molecular interactions of ribosomal components. II. Site-specific complex formation between 30S proteins and ribosomal RNA. Mol Gen Genet. 1971;112(1):1–8. doi: 10.1007/BF00266926. [DOI] [PubMed] [Google Scholar]
  13. Schaup H. W., Sogin M., Woese C. Characterization of an RNA "binding site" for a specific ribosomal protein of Escherichia coli. Mol Gen Genet. 1972;114(1):1–8. doi: 10.1007/BF00268740. [DOI] [PubMed] [Google Scholar]
  14. Sommer A., Traut R. R. Diagonal polyacrylamide-dodecyl sulfate gel electrophoresis for the identification of ribosomal proteins crosslinked with methyl-4-mercaptobutyrimidate. Proc Natl Acad Sci U S A. 1974 Oct;71(10):3946–3950. doi: 10.1073/pnas.71.10.3946. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Stöffler G., Daya L., Rak K. H., Garrett R. A. Ribosomal proteins. XXVI. The number of specific protein binding sites on 16 s and 23 s RNA of Escherichia coli. J Mol Biol. 1971 Dec 14;62(2):411–414. doi: 10.1016/0022-2836(71)90437-2. [DOI] [PubMed] [Google Scholar]
  16. Voynow P., Kurland C. G. Stoichiometry of the 30S ribosomal proteins of Escherichia coli. Biochemistry. 1971 Feb 2;10(3):517–524. doi: 10.1021/bi00779a026. [DOI] [PubMed] [Google Scholar]
  17. Wittmann H. G., Stöfflet G., Hindennach I., Kurland C. G., Birge E. A., Randall-Hazelbauer L., Nomura M., Kaltschmidt E., Mizushima S., Traut R. R. Correlation of 30S ribosomal proteins of Escherichia coli isolated in different laboratories. Mol Gen Genet. 1971;111(4):327–333. doi: 10.1007/BF00569784. [DOI] [PubMed] [Google Scholar]
  18. Zimmermann R. A., Garvin R. T., Gorini L. Alteration of a 30S ribosomal protein accompanying the ram mutation in Escherichia coli. Proc Natl Acad Sci U S A. 1971 Sep;68(9):2263–2267. doi: 10.1073/pnas.68.9.2263. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES