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. 1976 Jan;3(1):149–164. doi: 10.1093/nar/3.1.149

Cooperative binding of fluorescein-labeled clupeine by DNA.

K Wehling,, S Krauss, K G Wagner
PMCID: PMC342884  PMID: 1250694

Abstract

The alpha-amino group of clupeine fraction Z from herring sperm was coupled with fluorescein. Binding of the labeled protamine by DNA is accompanied by significant fluorescence quenching up to 80%. This allowed the convenient determination of the binding behavior of protamine and DNA. Binding was found to be strongly cooperative and not be significantly affected by the size of DNA. The ionic strength dependence in the range up to 0.3 M NaCl was rather small. Binding parameters were derived according to classical unique-site treatment and to a concept which includes vagrant multi-site binding.

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Selected References

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