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. 1976 Jan;3(1):261–276. doi: 10.1093/nar/3.1.261

Induced formation of covalent bonds between nucleoprotein components. V. UV or bisulfite induced polynucleotide-protein crosslinkage in bacteriophage MS2.

E I Budowsky, N A Simukova, M F Turchinsky, I V Boni, Y M Skoblov
PMCID: PMC342893  PMID: 1250703

Abstract

UV (lambda = 254 nm) irradiation of bacteriophage MS2 or its treatment with bisulfite induce covalent crosslinkage of the RNA to the coat protein. epilsonN-(2-oxopyrimidyl-4)-lysine was found in the phage hydrolysates after either type of treatment. An equimolar mixture of 0-methylhydroxylamine and bisulfite causes complete disappearance of the cross-links. This led to the conclusion that one of the factors responsible for the UV-induced polynucleotide-protein crosslinkage and the main factor in treatment with bisulfite is substitution of the exocyclic amino group of the activated cytosine nucleus by the lysine residue epilson-amino group of the protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boni I. V., Budowsky E. I. Transformation of non-covalent interactions in nucleoproteins into covalent bonds induced by nucleophilic reagents. I. The preparation and properties of the products of bisulfite ion-catalyzed reaction of amino acids and peptides with cytosine derivatives. J Biochem. 1973 Apr;73(4):821–830. doi: 10.1093/oxfordjournals.jbchem.a130145. [DOI] [PubMed] [Google Scholar]
  2. Budzik G. P., Lam S. S., Schoemaker H. J., Schimmel P. R. Two photo-cross-linked complexes of isoleucine specific transfer ribonucleic acid with aminoacyl transfer ribonucleic acid synthetases. J Biol Chem. 1975 Jun 25;250(12):4433–4439. [PubMed] [Google Scholar]
  3. Carpenter J. M., Kleczkowski A. The absence of photoreversible pyrimidine dimers in the RNA of ultraviolet-irradiated tobacco mosaic virus. Virology. 1969 Nov;39(3):542–547. doi: 10.1016/0042-6822(69)90101-9. [DOI] [PubMed] [Google Scholar]
  4. Furuse K., Watanabe I. Effects of ultraviolet light (UV) irradiation on RNA phage in H 2 O and in D 2 O. Virology. 1971 Oct;46(1):171–172. doi: 10.1016/0042-6822(71)90018-3. [DOI] [PubMed] [Google Scholar]
  5. Goddard J., Streeter D., Weber C., Gordon M. P. tudies on the inactivation of tobacco mosaic virus by ultraviolet light. Photochem Photobiol. 1966 Feb;5:213–222. doi: 10.1111/j.1751-1097.1966.tb05783.x. [DOI] [PubMed] [Google Scholar]
  6. Gorelic L. S., Lisagor P., Yang N. C. The photochemical reactions of 1,3-dimethyluracil with 1-aminopropane and poly-L-lysine. Photochem Photobiol. 1972 Dec;16(6):469–480. doi: 10.1111/j.1751-1097.1972.tb06315.x. [DOI] [PubMed] [Google Scholar]
  7. Gorelic L. Photoinduced convalent crosslinkage, in situ, of Escherichia coli 50 S ribosomal proteins to rRNA. Biochim Biophys Acta. 1975 May 1;390(2):209–225. doi: 10.1016/0005-2787(75)90342-1. [DOI] [PubMed] [Google Scholar]
  8. Hinnen R., Schäfer R., Franklin R. M. Structure and synthesis of a lipid-containing bacteriophage. Preparation of virus and localization of the structural proteins. Eur J Biochem. 1974 Dec 16;50(1):1–14. doi: 10.1111/j.1432-1033.1974.tb03867.x. [DOI] [PubMed] [Google Scholar]
  9. Hutchinson F. The lesions produced by ultraviolet light in DNA containing 5-bromouracil. Q Rev Biophys. 1973 May;6(2):201–246. doi: 10.1017/s0033583500001141. [DOI] [PubMed] [Google Scholar]
  10. Lin S. Y., Riggs A. D. Photochemical attachment of lac repressor to bromodeoxyuridine-substituted lac operator by ultraviolet radiation. Proc Natl Acad Sci U S A. 1974 Mar;71(3):947–951. doi: 10.1073/pnas.71.3.947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Markovitz A. Ultraviolet light-induced stable complexes of DNA and DNA polymerase. Biochim Biophys Acta. 1972 Nov 9;281(4):522–534. doi: 10.1016/0005-2787(72)90153-0. [DOI] [PubMed] [Google Scholar]
  12. Pörschke D. Analysis of a specific photoreaction in oligo- and polydeoxyadenylic acids. J Am Chem Soc. 1973 Dec 12;95(25):8440–8446. doi: 10.1021/ja00806a040. [DOI] [PubMed] [Google Scholar]
  13. Salomon J., Elad D. Selective photochemical alkylation of purines in DNA. Biochem Biophys Res Commun. 1974 Jun 4;58(3):890–894. doi: 10.1016/s0006-291x(74)80500-0. [DOI] [PubMed] [Google Scholar]
  14. Schoemaker H. J., Schimmel P. R. Photo-induced joining of a transfer RNA with its cognate aminoacyl-transfer RNA synthetase. J Mol Biol. 1974 Apr 25;84(4):503–513. doi: 10.1016/0022-2836(74)90112-0. [DOI] [PubMed] [Google Scholar]
  15. Schott H. N., Shetlar M. D. Photochemical addition of amino acids to thymine. Biochem Biophys Res Commun. 1974 Aug 5;59(3):1112–1116. doi: 10.1016/s0006-291x(74)80093-8. [DOI] [PubMed] [Google Scholar]
  16. Simukova N. A., Budowsky E. I. Conversion of non-covalent interactions in nucleoproteins into covalent bonds: UV-induced formation of polynucleotide-protein crosslinks in bacteriophage Sd virions. FEBS Lett. 1974 Jan 15;38(3):299–303. doi: 10.1016/0014-5793(74)80077-3. [DOI] [PubMed] [Google Scholar]
  17. Smith K. C., Aplin R. T. A mixed photoproduct of uracil and cysteine (5-S-cysteine-6-hydrouracil). A possible model for the in vivo cross-linking of deoxyribonucleic acid and protein by ultraviolet light. Biochemistry. 1966 Jun;5(6):2125–2130. doi: 10.1021/bi00870a046. [DOI] [PubMed] [Google Scholar]
  18. Smith K. C. Photochemical addition of amino acids to 14C-uracil. Biochem Biophys Res Commun. 1969 Feb 7;34(3):354–357. doi: 10.1016/0006-291x(69)90840-7. [DOI] [PubMed] [Google Scholar]
  19. Strniste G. F., Smith D. A. Induction of stable linkage between the deoxyribonucleic acid dependent ribonucleic acid polymerase and d(A-T)n-d(A-T)n by ultraviolet light. Biochemistry. 1974 Jan 29;13(3):485–493. doi: 10.1021/bi00700a014. [DOI] [PubMed] [Google Scholar]
  20. Tikchonenko T. I., Budowsky E. I., Sklyadneva V. B., Khromov I. S. The secondary structure of bacteriophage DNA in situ. 3. Reaction of Sd phage with O-methylhydroxylamine. J Mol Biol. 1971 Feb 14;55(3):535–547. doi: 10.1016/0022-2836(71)90335-4. [DOI] [PubMed] [Google Scholar]
  21. Tikchonenko T. I., Kisseleva N. P., Zintshenko A. I., Ulanov B. P., Budowsky E. I. Peculiarities of the secondary structure of bacteriophage DNA in situ. IV. Covalent cross-links between DNA and protein that arise in the reaction of S d phage with O-methylhydroxylamine. J Mol Biol. 1973 Jan;73(1):109–119. doi: 10.1016/0022-2836(73)90162-9. [DOI] [PubMed] [Google Scholar]
  22. Turchinsky M. F., Kusova K. S., Budowsky E. I. Conversion of non-covalent interactions in nucleoproteins into covalent bonds: bisulfite-induced formation of polynucleotide-protein crosslinks in MS2 bacteriophage virions. FEBS Lett. 1974 Jan 15;38(3):304–307. doi: 10.1016/0014-5793(74)80078-5. [DOI] [PubMed] [Google Scholar]
  23. Turchinsky M. F., Shershneva L. P. A simple method of quantitative recovery of nucleotides from thin layers. Anal Biochem. 1973 Aug;54(2):315–318. doi: 10.1016/0003-2697(73)90358-8. [DOI] [PubMed] [Google Scholar]
  24. Varghese A. J. Properties of photoaddition products of thymine and cysteine. Biochemistry. 1973 Jul 3;12(14):2725–2730. doi: 10.1021/bi00738a027. [DOI] [PubMed] [Google Scholar]
  25. Viñuela E., Algranati I. D., Ochoa S. Synthesis of virus-specific proteins in Escherichia coli infected with the RNA bacteriophage MS2. Eur J Biochem. 1967 Mar;1(1):3–11. doi: 10.1007/978-3-662-25813-2_2. [DOI] [PubMed] [Google Scholar]
  26. Werbin H., Valentine R. C., Hildalgo-Salvatierra O., McLaren A. D. Photobiology of RNA bacteriophages. II. U.V.-irradiation of f2: effects on extracellular stages of infection and on early replication. Photochem Photobiol. 1968 Mar;7(3):253–261. doi: 10.1111/j.1751-1097.1968.tb08014.x. [DOI] [PubMed] [Google Scholar]
  27. Yamada Y., Shigeta A., Nozu K. Ultraviolet effects on biological function of RNA phage MS2. Biochim Biophys Acta. 1973 Feb 23;299(1):121–135. doi: 10.1016/0005-2787(73)90403-6. [DOI] [PubMed] [Google Scholar]

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