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. 1976 Feb;3(2):465–476. doi: 10.1093/nar/3.2.465

Comparison of the fine structure of mitochondrial DNA from Saccharomyces cerevisiae and S. carlsbergensis: electron microscopy of partially denatured molecules.

G Christiansen, C Christiansen
PMCID: PMC342916  PMID: 768925

Abstract

Denaturation-maps of mitochondrial DNA from Saccharomyces cerevisiae and S. carlsbergensis have been derived from electron microscopic observations of partially denatured complete circular molecules and large fragments of these circles. The mitochondrial DNA from the two species differ by 6% in total length, but seems from the maps to contain some regions of apparent close homology. The cleavage pattern of the two DNAs by the restriction endonuclease EcoRI is compared by gel electrophoresis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bak A. L., Christiansen C., Christiansen G. Circular, repetitive DNA in yeast. Biochim Biophys Acta. 1972 May 29;269(3):527–530. doi: 10.1016/0005-2787(72)90144-x. [DOI] [PubMed] [Google Scholar]
  2. Bak A. L., Christiansen G., Christiansen C., Stenderup A., Orskov I., Orskov F. Circular DNA molecules controlling synthesis and transfer of the surface antigen (K88) in Escherichia coli. J Gen Microbiol. 1972 Nov;73(2):373–385. doi: 10.1099/00221287-73-2-373. [DOI] [PubMed] [Google Scholar]
  3. Bernardi G., Faures M., Piperno G., Slonimski P. P. Mitochondrial DNA's from respiratory-sufficient and cytoplasmic respiratory-deficient mutant yeast. J Mol Biol. 1970 Feb 28;48(1):23–42. doi: 10.1016/0022-2836(70)90216-0. [DOI] [PubMed] [Google Scholar]
  4. Blamire J., Cryer D. R., Finkelstein D. B., Marmur J. Sedimentation properties of yeast nuclear and mitochondrial DNA. J Mol Biol. 1972 Jun 14;67(1):11–24. doi: 10.1016/0022-2836(72)90382-8. [DOI] [PubMed] [Google Scholar]
  5. Christiansen C., Christiansen G., Bak A. L. Heterogeneity of mitochondrial DNA from Saccharomyces carlsbergensis: renaturation and sedimentation studies. J Mol Biol. 1974 Mar 25;84(1):65–82. doi: 10.1016/0022-2836(74)90212-5. [DOI] [PubMed] [Google Scholar]
  6. Christiansen G., Christiansen C., Bak A. L. Heterogeneity of mitochondrial DNA from Saccharomyces carlsbergensis. Denaturation mapping by electron microscopy. Nucleic Acids Res. 1975 Feb;2(2):197–210. doi: 10.1093/nar/2.2.197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Davis R. W., Hyman R. W. A study in evolution: the DNA base sequence homology between coliphages T7 and T3. J Mol Biol. 1971 Dec 14;62(2):287–301. doi: 10.1016/0022-2836(71)90428-1. [DOI] [PubMed] [Google Scholar]
  8. Griffin B. E., Fried M., Cowie A. Polyoma DNA: a physical map. Proc Natl Acad Sci U S A. 1974 May;71(5):2077–2081. doi: 10.1073/pnas.71.5.2077. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hollenberg C. P., Borst P., van Bruggen E. F. Mitochondrial DNA. V. A 25 micron closed circular duplex DNA molecule in wild-type yeast mitochondria. Stucture and genetic complexity. Biochim Biophys Acta. 1970 May 21;209(1):1–15. [PubMed] [Google Scholar]
  10. MACHATTIE L. A., BERNE K. I., THOMAS C. A., Jr ELECTRON MICROSCOPY OF DNA FROM HEMOPHILUS INFLUENZAE. J Mol Biol. 1965 Mar;11:648–649. doi: 10.1016/s0022-2836(65)80019-5. [DOI] [PubMed] [Google Scholar]
  11. Morimoto R., Lewin A., Hsu H. J., Rabinowitz M., Fukuhara H. Restriction endonuclease analysis of mitochondrial DNA from grande and genetically characterized cytoplasmic petite clones of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3868–3872. doi: 10.1073/pnas.72.10.3868. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Piperno G., Fonty G., Bernardi G. The mitochondrial genome of wild-type yeast cells. II. Investigations on the compositional heterogeneity of mitochondrial DNA. J Mol Biol. 1972 Mar 28;65(2):191–205. doi: 10.1016/0022-2836(72)90276-8. [DOI] [PubMed] [Google Scholar]
  13. Prunell A., Bernardi G. The mitochondrial genome of wild-type yeast cells. IV. Genes and spacers. J Mol Biol. 1974 Jul 15;86(4):825–841. doi: 10.1016/0022-2836(74)90356-8. [DOI] [PubMed] [Google Scholar]
  14. STUDIER F. W. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA. J Mol Biol. 1965 Feb;11:373–390. doi: 10.1016/s0022-2836(65)80064-x. [DOI] [PubMed] [Google Scholar]
  15. Sanders J. P., Flavell R. A., Borst P., Mol J. N. Nature of the base sequence conserved in the mitochondrial DNA of a low-density petite. Biochim Biophys Acta. 1973 Jul 13;312(3):441–457. doi: 10.1016/0005-2787(73)90443-7. [DOI] [PubMed] [Google Scholar]
  16. Sanders J. P., Heyting C., Borst P. The organization of genes in yeast mitochondrial DNA. I. The genes for large and small ribosomal RNA are far apart. Biochem Biophys Res Commun. 1975 Jul 22;65(2):699–707. doi: 10.1016/s0006-291x(75)80202-6. [DOI] [PubMed] [Google Scholar]
  17. Sharp P. A., Sugden B., Sambrook J. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry. 1973 Jul 31;12(16):3055–3063. doi: 10.1021/bi00740a018. [DOI] [PubMed] [Google Scholar]
  18. Young I. T., Levinstone D., Eden M., Tye B. K., Botstein D. Alignment of partial denaturation maps of circularly permuted DNA by computer. J Mol Biol. 1974 Jan 5;85(4):528–532. doi: 10.1016/0022-2836(74)90313-1. [DOI] [PubMed] [Google Scholar]

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