Abstract
A novel nucleoside, O2'-methylinosine (Im), has been identified as a constituent of the ribosomal RNA of Crithidia fasciculata, a hemoflaggelate protozoan. The nucleoside is released as part of an alkali-stable dinucleotide, Im-Up, by alkaline hydrolysis of Crithidia rRNA, and as a 5'-nucleotide, pIm, by snake venom hydrolysis of the same RNA. The Im-containing derivatives isolated from Crithidia rRNA were characterized by comparison with marker compounds prepared by chemical deamination of the corresponding adenosine analogues. O2'-Methylinosine prepared from either natural Im-Up or natural pIm had the same ultraviolet absorption spectra and chromatographic properties as marker Im. Characterization of the base and sugar components of Im as hypoxanthine and 2-O-methylribose, respectively, provided final confimration of structure. Control experiments have eliminated the possibility that Im arises from O2'-methyladenosine (Am), a known constituent of ribosomal RNA, by chemical or enzymatic deamination during hydrolysis of Crithidia rRNA.
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- Cunningham R. S., Gray M. W. Derivatives of N-(N-(9-beta-D-ribofuranosylpurin-6-yl)carbamoyl)threonine in phosphodiesterase hydrolysates of wheat embryo transfer ribonucleic acid. Biochemistry. 1974 Jan 29;13(3):543–553. doi: 10.1021/bi00700a022. [DOI] [PubMed] [Google Scholar]
- Gray M. W. Analysis of O2'-methylnucleoside 5'-phosphates in snake venom hydrolysates of RNA: identification of O2'-methyl-5-carboxymethyluridine as a constituent of yeast transfer RNA. Can J Biochem. 1975 Jul;53(7):735–746. doi: 10.1139/o75-101. [DOI] [PubMed] [Google Scholar]
- Gray M. W. Dinucleotide sequences containing both base and sugar modifications in the ribosomal RNA of Crithidia fasciculata. Biochim Biophys Acta. 1974 Dec 6;374(2):253–257. doi: 10.1016/0005-2787(74)90367-0. [DOI] [PubMed] [Google Scholar]
- Gray M. W. The presence of O-2'-methylpseudouridine in the 18S + 26S ribosomal ribonucleates of wheat embryo. Biochemistry. 1974 Dec 31;13(27):5453–5463. doi: 10.1021/bi00724a001. [DOI] [PubMed] [Google Scholar]
- LANE B. G., ALLEN F. W. The isolation of dinucleotides from alkali hydrolyzates of ribonucleates. Can J Biochem Physiol. 1961 Apr;39:721–728. doi: 10.1139/o61-073. [DOI] [PubMed] [Google Scholar]
- LANE B. G. The separation of adenosine, guanosine, cytidine and uridine by one-dimensional filter-paper chromatography. Biochim Biophys Acta. 1963 May 28;72:110–112. [PubMed] [Google Scholar]
- Maden B. E., Forbes J., de Jonge P., Klootwijk J. Presence of a hypermodified nucleotide in HeLa cell 18 S and Saccharomyces carlsbergensis 17 S ribosomal RNAs. FEBS Lett. 1975 Nov 1;59(1):60–63. doi: 10.1016/0014-5793(75)80341-3. [DOI] [PubMed] [Google Scholar]
- Nichols J. L., Lane B. G. Characterization of N4,O2'-dimethylcytidine, a rare nucleoside constituent of Escherichia coli 16-S RNA. Biochim Biophys Acta. 1968 Oct 29;166(3):605–615. doi: 10.1016/0005-2787(68)90367-5. [DOI] [PubMed] [Google Scholar]
- SINGH H., LANE B. G. THE ALKALI-STABLE DINUCLEOTIDE SEQUENCES IN 18S+28S RIBONUCLEATES FROM WHEAT GERM. Can J Biochem. 1964 Jul;42:1011–1021. doi: 10.1139/o64-112. [DOI] [PubMed] [Google Scholar]
- Saponara A. G., Enger M. D. The isolation from ribonucleic acid of substituted uridines containing alpha-aminobutyrate moieties derived from methionine. Biochim Biophys Acta. 1974 Apr 27;349(1):61–77. doi: 10.1016/0005-2787(74)90009-4. [DOI] [PubMed] [Google Scholar]
- Tazawa I., Tazawa S., Alderfer J. L., Ts'o P. O. A novel procedure for the synthesis of 2'-O-alkyl nucleotides. Biochemistry. 1972 Dec 19;11(26):4931–4937. doi: 10.1021/bi00776a009. [DOI] [PubMed] [Google Scholar]