Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1976 Apr;3(4):1065–1071. doi: 10.1093/nar/3.4.1065

On the use of ultraviolet light to study protein-DNA crosslinking.

J E Celis, M Fink, K Kaltoft
PMCID: PMC342965  PMID: 944918

Abstract

Irradiation of Ehrlich ascites chromatin with ultraviolet light (u.v.) leads to protein-DNA crosslinking as determined by CsCl isopycnic ultra-centrifugation and SDS-polyacrylamide gel electrophoresis. At the most 4.5% of the chromatin proteins labelled with (14C)-lysine and (14C)-arginine can be crosslinked to DNA at u.v. doses between 3.6 X 10(4) to 10.8 X 10(4) ergs/mm2. We find however that the crosslinking reaction is hindered by protein breakage induced by u.v. light of wave length of less than 2950 A. Our results point out that caution must be used in the interpretation of studies on protein-nucleic acid interactions using u.v. light.

Full text

PDF
1065

Images in this article

1067Image
on p.1067
1069Image
on p.1069

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALEXANDER P., MOROSON H. Cross-linking of deoxyribonucleic acid to protein following ultra-violet irradiation different cells. Nature. 1962 Jun 2;194:882–883. doi: 10.1038/194882a0. [DOI] [PubMed] [Google Scholar]
  2. Aktipis S., Iammartino A. J. Photochemical modification of aromatic residues in irradiated ribonuclease. Biochim Biophys Acta. 1972 Sep 29;278(2):239–242. doi: 10.1016/0005-2795(72)90227-9. [DOI] [PubMed] [Google Scholar]
  3. Han A., Korbelik M., Ban J. DNA-to-protein cross-linking in synchronized HeLa cells exposed to ultra-violet light. Int J Radiat Biol Relat Stud Phys Chem Med. 1975 Jan;27(1):63–74. doi: 10.1080/09553007514550061. [DOI] [PubMed] [Google Scholar]
  4. Hutchinson F. The lesions produced by ultraviolet light in DNA containing 5-bromouracil. Q Rev Biophys. 1973 May;6(2):201–246. doi: 10.1017/s0033583500001141. [DOI] [PubMed] [Google Scholar]
  5. Ilyin Y. V., Varshavsky A. Y., Mickelsaar U. N., Georgiev G. P. Studies on deoxyribonucleoprotein structure. Redistribution of proteins in mixtures of deoxyribonucleoproteins, DNA and RNA. Eur J Biochem. 1971 Sep 24;22(2):235–245. doi: 10.1111/j.1432-1033.1971.tb01537.x. [DOI] [PubMed] [Google Scholar]
  6. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  7. Schultz R. M., Iammartino A. J., Aktipis S. Circular dichroism study of the conformation of ultraviolet-irradiated ribonuclease A. Biochim Biophys Acta. 1975 Mar 28;386(1):120–128. doi: 10.1016/0005-2795(75)90252-4. [DOI] [PubMed] [Google Scholar]
  8. Smets L. A., Cornelis J. J. Repairable and irrepairable damage in 5-bromouracil-substituted DNA exposed to ultra-violet radiation. Int J Radiat Biol Relat Stud Phys Chem Med. 1971;19(5):445–457. doi: 10.1080/09553007114550581. [DOI] [PubMed] [Google Scholar]
  9. Vecchio G., Dose K., Salvatore G. Effects of ultraviolet irradiation (2537 A) on the structure of human thyroglobulin. Eur J Biochem. 1968 Aug;5(3):422–428. doi: 10.1111/j.1432-1033.1968.tb00386.x. [DOI] [PubMed] [Google Scholar]
  10. Weintraub H. The assembly of newly replicated DNA into chromatin. Cold Spring Harb Symp Quant Biol. 1974;38:247–256. doi: 10.1101/sqb.1974.038.01.028. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES