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. 1976 May;3(5):1151–1165. doi: 10.1093/nar/3.5.1151

Nuclear origin of specific yeast mitochondrial aminoacyl-tRNA synthetases.

J M Schneller, C Schneller, R Martin, A J Stahl
PMCID: PMC342977  PMID: 781620

Abstract

Hydroxylapatite chromatographies of mitochondrial and total enzymes from a rho+ yeast, or from the related rho degrees mitochondrial DNA-less mutant, show the occurrence in the mitochondrial enzyme of one Phe-, one Met-, one Leu-tRNA synthetase peak which elutes distinctly from the cytoplasmic counterpart and charges well mitochondrial tRNA, whereas the cytoplasmic enzyme does not. The measurement of the mitochondrial synthetases activities in various enzymatic extracts shows that they are not repressed in rho+ cells grown on 10% glucose and that they are concentrated in the mitochondria (Phe- and Met- tRNA synthetases) but are also present outside the mitochondria. It is concluded that yeast mitochondrial protein biosynthesis involves the nuclear coded mitochondrial specific Phe-, Met- and Leu-tRNA synthetases and that the entrance of the synthetases into the mitochondria needs no factor depending on the mitochondrial DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Accoceberry B., Schneller J. M., Stahl A. J. Etude comparative des valyl tRNA cytoplasmique et mitochondrial de levure. Biochimie. 1973;55(3):291–296. doi: 10.1016/s0300-9084(73)80128-2. [DOI] [PubMed] [Google Scholar]
  2. Accoceberry B., Stahl A. Chromatographic differences between the cytoplasmic and the mitochondrial t-RNA of Saccharomyces cerevisiae. Biochem Biophys Res Commun. 1971 Mar 19;42(6):1235–1243. doi: 10.1016/0006-291x(71)90038-6. [DOI] [PubMed] [Google Scholar]
  3. Accoceberry B., Stahl A. Séparation des membranes internes et externes de mitochondries de la levure Saccharomyces cerevisiae. C R Acad Sci Hebd Seances Acad Sci D. 1972 Jun 5;274(23):3135–3138. [PubMed] [Google Scholar]
  4. Barnett W. E., Brown D. H., Epler J. L. Mitochondrial-specific aminoacyl-RNA synthetases. Proc Natl Acad Sci U S A. 1967 Jun;57(6):1775–1781. doi: 10.1073/pnas.57.6.1775. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Boguslawski G., Vodkin M. H., Finkelstein D. B., Fink G. R. Histidyl-tRNAs and histidyl-tRNA synthetases in wild type and cytoplasmic petite mutants of Saccharomyces cerevisiae. Biochemistry. 1974 Oct 22;13(22):4659–4667. doi: 10.1021/bi00719a030. [DOI] [PubMed] [Google Scholar]
  6. Buck C. A., Nass M. M. Studies on mitochondrial tRNA from animal cells. I. A comparison of mitochondrial and cytoplasmic trna and aminoacyl-tRNA synthetases. J Mol Biol. 1969 Apr 14;41(1):67–82. doi: 10.1016/0022-2836(69)90126-0. [DOI] [PubMed] [Google Scholar]
  7. Casey J. W., Hsu H. J., Getz G. S., Rabinowitz M. Transfer RNA genes in mitochondrial DNA of grande (wild-type) yeast. J Mol Biol. 1974 Oct 5;88(4):735–747. doi: 10.1016/0022-2836(74)90396-9. [DOI] [PubMed] [Google Scholar]
  8. Chiu A. O., Suyama Y. Immunologic studies on intracellular isoenzymes; the mitochondrial and cytoplasmic leucyl-tRNA synthetases. Biochim Biophys Acta. 1973 Apr 11;299(4):557–563. doi: 10.1016/0005-2787(73)90227-x. [DOI] [PubMed] [Google Scholar]
  9. Chiu A. O., Suyama Y. The absence of structural relationship between mitochondrial and mitochondrial and cytoplasmic leucyl-tRNA synthetases from Tetrahymena pyriformis. Arch Biochem Biophys. 1975 Nov;171(1):43–54. doi: 10.1016/0003-9861(75)90005-3. [DOI] [PubMed] [Google Scholar]
  10. Fasiolo F., Befort N., Boulander Y., Ebel J. P. Purification et quelques propriétés de la phenylalanyl-tRNA synthetase de levure de boulangerie. Biochim Biophys Acta. 1970 Oct 15;217(2):305–318. [PubMed] [Google Scholar]
  11. Faye G., Kujawa C., Fukuhara H. Physical and genetic organization of petite and grande yeast mitochondrial DNA. IV. In vivo transcription products of mitochondrial DNA and localization of 23 S ribosomal RNA in petite mutants of saccharomyces cerevisiae. J Mol Biol. 1974 Sep 5;88(1):185–203. doi: 10.1016/0022-2836(74)90304-0. [DOI] [PubMed] [Google Scholar]
  12. Fukuhara H., Faye G., Michel F., Lazowska J., Deutsch J., Bolotin-Fukuhara M., slonimski P. P. Physical and genetic organization of petite and grande yeast mitochondrial DNA.I. Studies by RNA-DNA hybridization. Mol Gen Genet. 1974 May 31;130(3):215–238. doi: 10.1007/BF00268801. [DOI] [PubMed] [Google Scholar]
  13. Gross S. R., McCoy M. T., Gilmore E. B. Evidence for the involvement of a nuclear gene in the productin of the mitochondrial leucyl-tRNA synthetase of Neurospora. Proc Natl Acad Sci U S A. 1968 Sep;61(1):253–260. doi: 10.1073/pnas.61.1.253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kellems R. E., Allison V. F., Butow R. A. Cytoplasmic type 80S ribosomes associated with yeast mitochondria. IV. Attachment of ribosomes to the outer membrane of isolated mitochondria. J Cell Biol. 1975 Apr;65(1):1–14. doi: 10.1083/jcb.65.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Linnane A. W., Haslam J. M., Lukins H. B., Nagley P. The biogenesis of mitochondria in microorganisms. Annu Rev Microbiol. 1972;26:163–198. doi: 10.1146/annurev.mi.26.100172.001115. [DOI] [PubMed] [Google Scholar]
  16. Pearson R. L., Weiss J. F., Kelmers A. D. Improved separation of transfer RNA's on polychlorotrifuoroethylene-supported reversed-phase chromatography columns. Biochim Biophys Acta. 1971 Feb 11;228(3):770–774. doi: 10.1016/0005-2787(71)90748-9. [DOI] [PubMed] [Google Scholar]
  17. Reger B. J., Fairfield S. A., Epler J. L., Barnett W. E. Identification and origin of some chloroplast aminoacyl-tRNA synthetases and tRNAs. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1207–1213. doi: 10.1073/pnas.67.3.1207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schneller J. M., Martin R., Stahl A., Dirheimer G. Studies of odd bases in yeast mitochondrial tRNA: absense of the fluorescent "Y" base in mitochondrial DNA coded tRNAPhe, absence of 4-thiouridine. Biochem Biophys Res Commun. 1975 Jan 2;64(3):1046–1053. doi: 10.1016/0006-291x(75)90153-9. [DOI] [PubMed] [Google Scholar]
  19. Schneller J. M., Stahl A., Fukuhara H. Coding origin of isoaccepting tRNA in yeast mitochondria. Biochimie. 1975;57(9):1051–1057. doi: 10.1016/s0300-9084(75)80361-0. [DOI] [PubMed] [Google Scholar]
  20. Schneller J., Accoceberry B., Stahl A. J. Fractionation of yeast mitochondrial tRNA-Tyr and tRNA-Leu. FEBS Lett. 1975 Apr 15;53(1):44–48. doi: 10.1016/0014-5793(75)80678-8. [DOI] [PubMed] [Google Scholar]

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