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. 1976 May;3(5):1203–1213. doi: 10.1093/nar/3.5.1203

Stimulation of transcription on chromatin by polar organic compounds.

W H Strätling
PMCID: PMC342980  PMID: 781622

Abstract

Polar organic compounds, including DMSO, increase RNA synthesis on isolated chromatin by E. coli RNA polymerase and RNA polymerase II from calf thymus. Transcription is stimulated on chromatin from Friend-virus-infected erythroleukemia cells and from various other sources. Using procedures which inhibit specifically the formation of a stable initiation complex, it is shown that the stimulation does not result from an increase in initiation of both E. coli and the eukaryotic RNA polymerase. After separation of chromatin into template active and inactive fractions, DMSO increases RNA synthesis by a factor of about 1.5 using the template inactive fraction, while stimulation of transcription on the template active portion is lower (factor of 1.2). It is suggested that the effect on RNA synthesis is mediated by a weakening of the apolar interactions between histones in chromatin subunits, releasing transcription partially from the constraints imposed by histones.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baldwin J. P., Boseley P. G., Bradbury E. M., Ibel K. The subunit structure of the eukaryotic chromosome. Nature. 1975 Jan 24;253(5489):245–249. doi: 10.1038/253245a0. [DOI] [PubMed] [Google Scholar]
  2. Bradbury E. M., Rattle H. W. Simple computer-aided approach for the analyses of the nuclear-magnetic-resonance spectra of histones. Fractions F1, Fsa1, F2B, cleaved halves of F2B and F2B-DNA. Eur J Biochem. 1972 May 23;27(2):270–281. doi: 10.1111/j.1432-1033.1972.tb01836.x. [DOI] [PubMed] [Google Scholar]
  3. Chevaillier P., Philippe M. Aspects structuraux et biochimiques des effets de quelques milieux utilisés pour l'isolement et le fractionnement du noyau de foie de souris. Exp Cell Res. 1973 Nov;82(1):1–14. doi: 10.1016/0014-4827(73)90238-3. [DOI] [PubMed] [Google Scholar]
  4. Friend C., Scher W., Holland J. G., Sato T. Hemoglobin synthesis in murine virus-induced leukemic cells in vitro: stimulation of erythroid differentiation by dimethyl sulfoxide. Proc Natl Acad Sci U S A. 1971 Feb;68(2):378–382. doi: 10.1073/pnas.68.2.378. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HSU T. C. Differential rate in RNA synthesis between euchromatin and heterochromatin. Exp Cell Res. 1962 Aug;27:332–334. doi: 10.1016/0014-4827(62)90238-0. [DOI] [PubMed] [Google Scholar]
  6. Ingles C. J. Antigenic homology of eukaryotic RNA polymerases. Biochem Biophys Res Commun. 1973 Nov 16;55(2):364–371. doi: 10.1016/0006-291x(73)91096-6. [DOI] [PubMed] [Google Scholar]
  7. Kornberg R. D. Chromatin structure: a repeating unit of histones and DNA. Science. 1974 May 24;184(4139):868–871. doi: 10.1126/science.184.4139.868. [DOI] [PubMed] [Google Scholar]
  8. Langmore J. P., Wooley J. C. Chromatin architecture: investigation of a subunit of chromatin by dark field electron microscopy. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2691–2695. doi: 10.1073/pnas.72.7.2691. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Levy J., Terada M., Rifkind R. A., Marks P. A. Induction of erythroid differentiation by dimethylsulfoxide in cells infected with Friend virus: relationship to the cell cycle. Proc Natl Acad Sci U S A. 1975 Jan;72(1):28–32. doi: 10.1073/pnas.72.1.28. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nakanishi S., Adhya S., Gottesman M., Pastan I. Activation of transcription at specific promoters by glycerol. J Biol Chem. 1974 Jul 10;249(13):4050–4056. [PubMed] [Google Scholar]
  11. Noll M. Internal structure of the chromatin subunit. Nucleic Acids Res. 1974 Nov;1(11):1573–1578. doi: 10.1093/nar/1.11.1573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Olins A. L., Olins D. E. Spheroid chromatin units (v bodies). Science. 1974 Jan 25;183(4122):330–332. doi: 10.1126/science.183.4122.330. [DOI] [PubMed] [Google Scholar]
  13. Ostertag W., Melderis H., Steinheider G., Kluge N., Dube S. Synthesis of mouse haemoglobin and globin mRNA in leukaemic cell cultures. Nat New Biol. 1972 Oct 25;239(95):231–234. doi: 10.1038/newbio239231a0. [DOI] [PubMed] [Google Scholar]
  14. Reeck G. R., Simpson R. T., Sober H. A. Resolution of a spectrum of nucleoprotein species in sonicated chromatin. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2317–2321. doi: 10.1073/pnas.69.8.2317. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ross J., Ikawa Y., Leder P. Globin messenger-RNA induction during erythroid differentiation of cultured leukemia cells. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3620–3623. doi: 10.1073/pnas.69.12.3620. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. So A. G., Downey K. M. Studies on the mechanism of ribonucleic acid synthesis. II. Stabilization of the deoxyribonucleic acid-ribonucleic acid polymerase complex by the formation of a single phosphodiester bond. Biochemistry. 1970 Nov 24;9(24):4788–4793. doi: 10.1021/bi00826a024. [DOI] [PubMed] [Google Scholar]
  17. Tanaka M., Levy J., Terada M., Breslow R., Rifkind R. A., Marks P. A. Induction of erythroid differentiation in murine virus infected eythroleukemia cells by highly polar compounds. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1003–1006. doi: 10.1073/pnas.72.3.1003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Travers A. On the nature of DNA promoter conformations. The effects of glycerol and dimethylsulphoxide. Eur J Biochem. 1974 Sep 16;47(3):435–441. doi: 10.1111/j.1432-1033.1974.tb03710.x. [DOI] [PubMed] [Google Scholar]
  19. Tsai M-J, Schwartz R. J., Tsai S. Y., O'Malley B. W. Effects of estrogen on gene expression in the chick oviduct. IV. Initiation of RNA synthesis on DNA and chromatin. J Biol Chem. 1975 Jul 10;250(13):5165–5174. [PubMed] [Google Scholar]
  20. Van Holde K. E., Sahasrabuddhe C. G., Shaw B. R. A model for particulate structure in chromatin. Nucleic Acids Res. 1974 Nov;1(11):1579–1586. doi: 10.1093/nar/1.11.1579. [DOI] [PMC free article] [PubMed] [Google Scholar]

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