Abstract
The synthesis of serum albumin in an in vitro protein-synthesizing system from wheat germ stimulated with rat liver polysomal RNA is demonstrated by immunoprecipitation. The newly synthesized albumin has the same electrophoretic mobility as rat serum albumin. There is a linear increase in precursor incorporation into total protein and albumin with increasing RNA concentration. Potassium and magnesium optima for albumin synthesis are different from those for total protein synthesis.
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- DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
- Faber A. J., Miall S. H., Tamaoki T. Synthesis of albumin with exogenous mouse-liver messenger RNA in a homologous cell-free system. Can J Biochem. 1974 May;52(5):429–432. doi: 10.1139/o74-064. [DOI] [PubMed] [Google Scholar]
- Gozes I., Schmitt H., Littauer U. Z. Translation in vitro of rat brain messenger RNA coding for tubulin and actin. Proc Natl Acad Sci U S A. 1975 Feb;72(2):701–705. doi: 10.1073/pnas.72.2.701. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kemper B., Habener J. F., Mulligan R. C., Potts J. T., Jr, Rich A. Pre-proparathyroid hormone: a direct translation product of parathyroid messenger RNA. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3731–3735. doi: 10.1073/pnas.71.9.3731. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Marcu K., Dudock B. Characterization of a highly efficient protein synthesizing system derived from commercial wheat germ. Nucleic Acids Res. 1974 Nov;1(11):1385–1397. doi: 10.1093/nar/1.11.1385. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Marcus A., Efron D., Weeks D. P. The wheat embryo cell-free system. Methods Enzymol. 1974;30:749–754. doi: 10.1016/0076-6879(74)30073-0. [DOI] [PubMed] [Google Scholar]
- McKeehan W. L. Regulation of hemoglobin synthesis. Effect of concentration of messenger ribonucleic acid, ribosome subunits, initiation factors, and salts on ratio of alpha and beta chains synthesized in vitro. J Biol Chem. 1974 Oct 25;249(20):6517–6526. [PubMed] [Google Scholar]
- Palmiter R. D. Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid. Biochemistry. 1974 Aug 13;13(17):3606–3615. doi: 10.1021/bi00714a032. [DOI] [PubMed] [Google Scholar]
- Peters T., Jr, Peters J. C. The biosynthesis of rat serum albumin. VI. Intracellular transport of albumin and rates of albumin and liver protein synthesis in vivo under various physiological conditions. J Biol Chem. 1972 Jun 25;247(12):3858–3863. [PubMed] [Google Scholar]
- Peters T., Jr Serum albumin. Adv Clin Chem. 1970;13:37–111. doi: 10.1016/s0065-2423(08)60385-6. [DOI] [PubMed] [Google Scholar]
- Peterson J. A. Discontinuous variability, in the form of a geometric progression, of albumin production in hepatoma and hybrid cells. Proc Natl Acad Sci U S A. 1974 May;71(5):2062–2066. doi: 10.1073/pnas.71.5.2062. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roberts B. E., Paterson B. M. Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2330–2334. doi: 10.1073/pnas.70.8.2330. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rotermund H. M., Schreiber G., Maeno H., Weinssen U., Weigand K. The ratio of albumin synthesis to total protein synthesis in normal rat liver, in host liver, and in Morris hepatoma 9121. Cancer Res. 1970 Aug;30(8):2139–2146. [PubMed] [Google Scholar]
- Schreiber G., Rotermund H. M., Maeno H., Weigand K., Lesch R. The proption of the incorporation of leucine into albumin to that into totaprotein in rat liver and hepatoma Morris 5123 TC. Eur J Biochem. 1969 Sep;10(2):355–361. doi: 10.1111/j.1432-1033.1969.tb00698.x. [DOI] [PubMed] [Google Scholar]
- Shafritz D. A. Protein synthesis with messenger ribonucleic acid fractions from membrane-bound and free liver polysomes. Translation characteristics of liver polysomal ribonucleic acids and evidence for albumin production in a messenger-dependent reticulocyte cell-free system. J Biol Chem. 1974 Jan 10;249(1):81–88. [PubMed] [Google Scholar]
- Shih D. S., Kaesberg P. Translation of brome mosaic viral ribonucleic acid in a cell-free system derived from wheat embryo. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1799–1803. doi: 10.1073/pnas.70.6.1799. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taylor J. M., Schimke R. T. Synthesis of rat liver albumin in a rabbit reticulocyte cell-free protein-synthesizing system. J Biol Chem. 1973 Nov 25;248(22):7661–7668. [PubMed] [Google Scholar]
- Verma D. P., Nash D. T., Schulman H. M. Isolation and in vitro translation of soybean leghaemoglobin mRNA. Nature. 1974 Sep 6;251(5470):74–77. doi: 10.1038/251074a0. [DOI] [PubMed] [Google Scholar]