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. 1976 Jul;3(7):1747–1760. doi: 10.1093/nar/3.7.1747

Two restriction endonucleases from Bacillus globiggi.

V Pirrotta
PMCID: PMC343033  PMID: 967673

Abstract

The sites of action of the restriction enzyme Bgl II on lambda DNA are mapped. This enzyme recognises the sequence 5' ...AGATCT...3' and makes staggered cuts producing sticky ends. In lambda DNA, the second A in this sequence is methylated about 50% of the time by a bacterial methylase absent in E. coli dam. In contrast to Bgl II, Bgl I makes many cuts in lambda DNA and produces 5' terminals which are not substrates for polynucleotide kinase.

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Selected References

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