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. 1976 Aug;3(8):1925–1936. doi: 10.1093/nar/3.8.1925

Preparation and properties of a poly A resin and its use in the isolation of naturally occurring poly U sequences

S Venkatesan 1, Hiroshi Nakazato 1, Mary Edmonds 1
PMCID: PMC343049  PMID: 967682

Abstract

A method for the covalent attachment of poly A, as well as other nucleic acids and nucleosides, to a methylene dianiline derivative of starch is described. The properties of this poly A resin and its use for the recovery of poly U sequences from both nuclear and cytoplasmic extracts of HeLa cells is described.

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Selected References

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  1. BAR-ELI A., KATCHALSKI E. Preparation and properties of water-insoluble derivatives of trypsin. J Biol Chem. 1963 May;238:1690–1698. [PubMed] [Google Scholar]
  2. Campbell D. H., Luescher E., Lerman L. S. Immunologic Adsorbents: I. Isolation of Antibody by Means of a Cellulose-Protein Antigen. Proc Natl Acad Sci U S A. 1951 Sep;37(9):575–578. doi: 10.1073/pnas.37.9.575. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Edmonds M., Vaughan M. H., Jr, Nakazato H. Polyadenylic acid sequences in the heterogeneous nuclear RNA and rapidly-labeled polyribosomal RNA of HeLa cells: possible evidence for a precursor relationship. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1336–1340. doi: 10.1073/pnas.68.6.1336. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. GRUBHOFER N., SCHLEITH L. Die Kupplung von Proteinen an diazotiertes Polyaminostyrol. Hoppe Seylers Z Physiol Chem. 1954;297(2):108–112. [PubMed] [Google Scholar]
  5. Goldstein L., Pecht M., Blumberg S., Atlas D., Levin Y. Water-insoluble enzymes. Synthesis of a new carrier and its utilization for preparation of insoluble derivatives of papain, trypsin, and subtilopeptidase A. Biochemistry. 1970 May 26;9(11):2322–2334. doi: 10.1021/bi00813a016. [DOI] [PubMed] [Google Scholar]
  6. Molloy G. R., Thomas W. L., Darnell J. E. Occurrence of uridylate-rich oligonucleotide regions in heterogeneous nuclear RNA of HeLa cells. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3684–3688. doi: 10.1073/pnas.69.12.3684. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Nakazato H., Edmonds M. Purification of messenger RNA and heterogeneous nuclear RNA containing poly(a) sequences. Methods Enzymol. 1974;29:431–443. doi: 10.1016/0076-6879(74)29035-9. [DOI] [PubMed] [Google Scholar]
  8. SEBRING E. D., SALZMAN N. P. AN IMPROVED PROCEDURE FOR MEASURING THE DISTRIBUTION OF P32O4--AMONG THE NUCLEOTIDES OF RIBONUCLEIC ACID. Anal Biochem. 1964 May;8:126–129. doi: 10.1016/0003-2697(64)90177-0. [DOI] [PubMed] [Google Scholar]
  9. Sundaram P. V. Some general methods of preparing affinity columns. Nucleic Acids Res. 1974 Nov;1(11):1587–1599. doi: 10.1093/nar/1.11.1587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Wagner A. F., Bugianesi R. L., Shen T. Y. Preparation of sepharose-bound poly (rI:rC). Biochem Biophys Res Commun. 1971 Oct 1;45(1):184–189. doi: 10.1016/0006-291x(71)90067-2. [DOI] [PubMed] [Google Scholar]
  11. Zimmerman S. B. Precipitation of polynucleotides from acidic solution. Effects of base composition and ionic strength. Biochemistry. 1973 Jul 17;12(15):2916–2925. doi: 10.1021/bi00739a023. [DOI] [PubMed] [Google Scholar]

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