Figure 2 .

Depletion of yeast essential genes results in elevated levels of illegitimate mating. (A) MATα Tet alleles were grown on YPD or YPD containing doxycycline (10 μg/ml) for 24 hr and a standard mating test was performed using MATα and MATa tester strains. Representative images of strains with elevated levels of illegitimate diploid formation following growth in doxycycline are shown. (B) The resulting number of illegitimate diploid colonies that grew without doxycycline treatment was subtracted from the number that grew with doxycycline treatment and was used to subcategorize the strains into four groups. For each group, the distribution of percentage of budded cells with Ddc2-YFP foci was plotted. Bold lines represent the median values, boxes represent the upper and lower quartiles, whiskers represent 1.5 times the interquartile range, and outliers are indicated by circles. (C) Comparison of Tet alleles with elevated levels of Ddc2 foci and >10 illegitimate mating diploid colonies.