Skip to main content
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1976 Aug;3(8):1997–2003. doi: 10.1093/nar/3.8.1997

The conservation of poly-A-containing RNA during the dormant state of the moss Polytrichum commune.

G Seibert, K Loris, J Zöllner, B Frenzel, R K Zahn
PMCID: PMC343055  PMID: 967684

Abstract

The moss Polytrichum commune can be dried to less than 2% of free water and kept for some weeks without losing its viability. Upon rehydration of the moss, protein synthesis starts about 60 minutes before incorporation of radioactive precursors into RNA can be observed. Pulse labeled total RNA and poly-A-containing RNA do not show quantitative or qualitative alterations during desiccation up to 20 days.

Full text

PDF
1997

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aronson A. Membrane-bound messenger RNA and polysomes in sporulating bacteria. J Mol Biol. 1965 Aug;13(1):92–104. doi: 10.1016/s0022-2836(65)80082-1. [DOI] [PubMed] [Google Scholar]
  2. Aviv H., Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. doi: 10.1073/pnas.69.6.1408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berger S. L., Cooper H. L. Very short-lived and stable mRNAs from resting human lymphocytes. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3873–3877. doi: 10.1073/pnas.72.10.3873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chen D., Sarid S., Katchalski E. The role of water stress in the inactivation of messenger RNA of germinating wheat embryos. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1378–1383. doi: 10.1073/pnas.61.4.1378. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cupello A., Hydén H. Separation of brain-RNA by micro-electrophoresis on agarose-acrylamide gels. Neurobiology. 1975 Jun;5(3):129–136. [PubMed] [Google Scholar]
  6. Dahlberg A. E., Dingman C. W., Peacock A. C. Electrophoretic characterization of bacterial polyribosomes in agarose-acrylamide composite gels. J Mol Biol. 1969 Apr 14;41(1):139–147. doi: 10.1016/0022-2836(69)90131-4. [DOI] [PubMed] [Google Scholar]
  7. Dhindsa R. S., Bewley J. D. Plant desiccation: polysome loss not due to ribonuclease. Science. 1976 Jan 16;191(4223):181–182. doi: 10.1126/science.1246604. [DOI] [PubMed] [Google Scholar]
  8. Hsiao T. C. Rapid Changes in Levels of Polyribosomes in Zea mays in Response to Water Stress. Plant Physiol. 1970 Aug;46(2):281–285. doi: 10.1104/pp.46.2.281. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lee S. Y., Mendecki J., Brawerman G. A polynucleotide segment rich in adenylic acid in the rapidly-labeled polyribosomal RNA component of mouse sarcoma 180 ascites cells. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1331–1335. doi: 10.1073/pnas.68.6.1331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lim L., Canellakis E. S. Adenine-rich polymer associated with rabbit reticulocyte messenger RNA. Nature. 1970 Aug 15;227(5259):710–712. doi: 10.1038/227710a0. [DOI] [PubMed] [Google Scholar]
  11. Lindberg U., Persson T. Isolation of mRNA from KB-cells by affinity chromatography on polyuridylic acid covalently linked to Sepharose. Eur J Biochem. 1972 Dec 4;31(2):246–254. doi: 10.1111/j.1432-1033.1972.tb02527.x. [DOI] [PubMed] [Google Scholar]
  12. Mendecki J., Lee S. Y., Brawerman G. Characteristics of the polyadenylic acid segment associated with messenger ribonucleic acid in mouse sarcoma 180 ascites cells. Biochemistry. 1972 Feb 29;11(5):792–798. doi: 10.1021/bi00755a018. [DOI] [PubMed] [Google Scholar]
  13. Pemberton R. E., Liberti P., Baglioni C. Isolation of messenger RNA from polysomes by chromatography on oligo(dT)-cellulose. Anal Biochem. 1975 May 26;66(1):18–28. doi: 10.1016/0003-2697(75)90720-4. [DOI] [PubMed] [Google Scholar]
  14. Vassart G., Brocas H., Nokin P., Dumont J. E. Translation in Xenopus oocytes of thyroglobulin mRNA isolated by poly(U)-Sepharose affinity chromatography. Biochim Biophys Acta. 1973 Nov 14;324(4):575–580. doi: 10.1016/0005-2787(73)90219-0. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES