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. 1976 Sep;3(9):2277–2291. doi: 10.1093/nar/3.9.2277

Satellite DNA sequence content of polylysine-titratable and nuclease-resistant fractions of mouse liver hepatoma chromatin.

J D Duerksen, I J Paul
PMCID: PMC343083  PMID: 184439

Abstract

Micrococcal nuclease digestion of mouse TLT liver hepatoma chromatin proceeds rapidly to the point where approximately 35% of the DNA is recoverable by centrifugation of the chromatin DNA through 3M CsCl. The satellite DNA sequence content of this recoverable DNA is the same as whole chromatin DNA (10%). The 11s (penultimate digestion product) monomer, as well as intermediate multiples and relatively undigested large chromatin segments are separable on steep hlycerol gradients. The DNA isolated from these fractions also contains the normal 10% satellite DNA content. Progressive polylysine titration of chromatin followed by nuclease digestion gives anomalous recoveries of DNA but, nonetheless, the satellite sequence content titration of chromatin, followed by pronase and then nuclease digestion, again gave recoverable DNA with a satellite sequence content of 10%. These results are discussed in terms of the conclusion that nucleosome (or upsilon-body) structures are distributed in a random fashion over the genome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnold E. A., Wahn U., Young K. E. Isolation and characterization of the DNA fraction of rat liver chromatin which binds polylysine. Nucleic Acids Res. 1975 May;2(5):667–681. doi: 10.1093/nar/2.5.667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Arnold E. A., Young K. E. Heterogeneity of chromatin: fractionation of sonicated rat liver chromatin by partial precipitation with Mg2+. Arch Biochem Biophys. 1974 Sep;164(1):73–89. doi: 10.1016/0003-9861(74)90009-5. [DOI] [PubMed] [Google Scholar]
  3. Axel R., Cedar H., Felsenfeld G. Chromatin template activity and chromatin structure. Cold Spring Harb Symp Quant Biol. 1974;38:773–783. doi: 10.1101/sqb.1974.038.01.082. [DOI] [PubMed] [Google Scholar]
  4. Axel R., Cedar H., Felsenfeld G. Synthesis of globin ribonucleic acid from duck-reticulocyte chromatin in vitro. Proc Natl Acad Sci U S A. 1973 Jul;70(7):2029–2032. doi: 10.1073/pnas.70.7.2029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Axel R., Cedar H., Felsenfield G. The structure of the globin genes in chromatin. Biochemistry. 1975 Jun 3;14(11):2489–2495. doi: 10.1021/bi00682a031. [DOI] [PubMed] [Google Scholar]
  6. Axel R. Cleavage of DNA in nuclei and chromatin with staphylococcal nuclease. Biochemistry. 1975 Jul;14(13):2921–2925. doi: 10.1021/bi00684a020. [DOI] [PubMed] [Google Scholar]
  7. Clark R. J., Felsenfeld G. Chemical probes of chromatin structure. Biochemistry. 1974 Aug 13;13(17):3622–3628. doi: 10.1021/bi00714a034. [DOI] [PubMed] [Google Scholar]
  8. Clark R. J., Felsenfeld G. Structure of chromatin. Nat New Biol. 1971 Jan 27;229(4):101–106. doi: 10.1038/newbio229101a0. [DOI] [PubMed] [Google Scholar]
  9. Duerksen J. D., McCarthy B. J. Distribution of deoxyribonucleic acid sequences in fractionated chromatin. Biochemistry. 1971 Apr 13;10(8):1471–1478. doi: 10.1021/bi00784a031. [DOI] [PubMed] [Google Scholar]
  10. Finch J. T., Noll M., Kornberg R. D. Electron microscopy of defined lengths of chromatin. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3320–3322. doi: 10.1073/pnas.72.9.3320. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gorovsky M. A., Keevert J. B. Subunit structure of a naturally occurring chromatin lacking histones F1 and F3. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3536–3540. doi: 10.1073/pnas.72.9.3536. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Itzhaki R. F. Accessible DNA in chromatin. Eur J Biochem. 1974 Aug 15;47(1):27–33. doi: 10.1111/j.1432-1033.1974.tb03664.x. [DOI] [PubMed] [Google Scholar]
  13. Itzhaki R. F. Studies on the accessibility of deoxyribonucleic acid in deoxyribonucleoprotein to cationic molecules. Biochem J. 1971 May;122(4):583–592. doi: 10.1042/bj1220583. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kongsvik J. R., Messineo L. The isolation and characterization of deoxyribonucleoproteins from human spleen. Arch Biochem Biophys. 1970 Jan;136(1):160–166. doi: 10.1016/0003-9861(70)90337-1. [DOI] [PubMed] [Google Scholar]
  15. Kornberg R. D., Thomas J. O. Chromatin structure; oligomers of the histones. Science. 1974 May 24;184(4139):865–868. doi: 10.1126/science.184.4139.865. [DOI] [PubMed] [Google Scholar]
  16. Lacy E., Axel R. Analysis of DNA of isolated chromatin subunits. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3978–3982. doi: 10.1073/pnas.72.10.3978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Li H. J., Chang C. Polylysine binding to histone-bound regions in chromatin. Biochem Biophys Res Commun. 1972 May 26;47(4):883–887. doi: 10.1016/0006-291x(72)90575-x. [DOI] [PubMed] [Google Scholar]
  18. McGhee J. D., Kimmel C. B. Evidence for a subunit structure of chromatin in mouse myeloma cells. Chromosoma. 1975 Sep 26;52(2):189–205. doi: 10.1007/BF00326267. [DOI] [PubMed] [Google Scholar]
  19. Murphy E. C., Jr, Hall S. H., Shepherd J. H., Weiser R. S. Fractionation of mouse myeloma chromatin. Biochemistry. 1973 Sep 25;12(20):3843–3853. doi: 10.1021/bi00744a008. [DOI] [PubMed] [Google Scholar]
  20. Noll M. Internal structure of the chromatin subunit. Nucleic Acids Res. 1974 Nov;1(11):1573–1578. doi: 10.1093/nar/1.11.1573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Noll M. Subunit structure of chromatin. Nature. 1974 Sep 20;251(5472):249–251. doi: 10.1038/251249a0. [DOI] [PubMed] [Google Scholar]
  22. Oosterhof D. K., Hozier J. C., Rill R. L. Nucleas action on chromatin: evidence for discrete, repeated nucleoprotein units along chromatin fibrils. Proc Natl Acad Sci U S A. 1975 Feb;72(2):633–637. doi: 10.1073/pnas.72.2.633. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Oudet P., Gross-Bellard M., Chambon P. Electron microscopic and biochemical evidence that chromatin structure is a repeating unit. Cell. 1975 Apr;4(4):281–300. doi: 10.1016/0092-8674(75)90149-x. [DOI] [PubMed] [Google Scholar]
  24. Pardue M. L., Gall J. G. Chromosomal localization of mouse satellite DNA. Science. 1970 Jun 12;168(3937):1356–1358. doi: 10.1126/science.168.3937.1356. [DOI] [PubMed] [Google Scholar]
  25. Paul I. J., Duerksen J. D. Release of euchromatic segments by Mg/Ca-dependent autodigestion of chromatin. Biochem Biophys Res Commun. 1976 Jan 12;68(1):97–105. doi: 10.1016/0006-291x(76)90015-2. [DOI] [PubMed] [Google Scholar]
  26. Paul J., Duerksen J. D. Chromatin-associated RNA content of heterochromatin and euchromatin. Mol Cell Biochem. 1975 Oct 31;9(1):9–16. doi: 10.1007/BF01731728. [DOI] [PubMed] [Google Scholar]
  27. Sahasrabuddhe C. G., Van Holde K. E. The effect of trypsin on nuclease-resistant chromatin fragments. J Biol Chem. 1974 Jan 10;249(1):152–156. [PubMed] [Google Scholar]
  28. Shaw B. R., Corden J. L., Sahasrabuddhe C. G., Van Holde K. E. Chromatographic separation of chromatin subunits. Biochem Biophys Res Commun. 1974 Dec 23;61(4):1193–1198. doi: 10.1016/s0006-291x(74)80410-9. [DOI] [PubMed] [Google Scholar]
  29. Shaw B. R., Herman T. M., Kovacic R. T., Beaudreau G. S., Van Holde K. E. Analysis of subunit organization in chicken erythrocyte chromatin. Proc Natl Acad Sci U S A. 1976 Feb;73(2):505–509. doi: 10.1073/pnas.73.2.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Simpson R. T. Interaction of a repotter molecule with chromatin. Evidence suggesting that the proteins of chromatin do not occupy the minor groove of deoxyribonucleic acid. Biochemistry. 1970 Nov 24;9(24):4814–4819. doi: 10.1021/bi00826a028. [DOI] [PubMed] [Google Scholar]
  31. Simpson R. T., Polacow I. Protein-DNA interactions in extended and condensed chromatin. Biochem Biophys Res Commun. 1973 Dec 19;55(4):1078–1084. doi: 10.1016/s0006-291x(73)80005-1. [DOI] [PubMed] [Google Scholar]
  32. Sollner-Webb B., Felsenfeld G. A comparison of the digestion of nuclei and chromatin by staphylococcal nuclease. Biochemistry. 1975 Jul;14(13):2915–2920. doi: 10.1021/bi00684a019. [DOI] [PubMed] [Google Scholar]
  33. Varshavsky A. J., Ilyin Y. V., Georgiev G. P. Very long stretches of free DNA in chromatin. Nature. 1974 Aug 16;250(467):602–606. doi: 10.1038/250602a0. [DOI] [PubMed] [Google Scholar]
  34. Yunis J. J., Yasmineh W. G. Satellite DNA in constitutive heterochromatin of the guinea pig. Science. 1970 Apr 10;168(3928):263–265. doi: 10.1126/science.168.3928.263. [DOI] [PubMed] [Google Scholar]
  35. de Pomerai D. I., Chesterton C. J., Butterworth P. H. Preparation of chromatin. Variation in the template properties of chromatin dependent on the method of perparation. Eur J Biochem. 1974 Aug 1;46(3):461–471. doi: 10.1111/j.1432-1033.1974.tb03639.x. [DOI] [PubMed] [Google Scholar]

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