Abstract
The 5' nucleotides of the double-stranded RNAs of yeast killer factor have been isolated by digestion with pancreatic, T1 and T2 RNase followed by two-dimensional electrophoresis. They were identified by bacterial alkaline phosphatase and snake venom phosphodiesterase digestions. Both the larger double-stranded RNA (L, of 2.5 x 10(6) daltons) and the smaller double-stranded RNA (M, of 1.4 x 10(6) daltons) have the 5' end groups pppGp. These 5' ends are dissimilar to those of the double-stranded RNAs of animal viruses but may be characteristic of the 5' ends of the double-stranded RNAs of fungal viruses.
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- Adams J. M., Cory S. Modified nucleosides and bizarre 5'-termini in mouse myeloma mRNA. Nature. 1975 May 1;255(5503):28–33. doi: 10.1038/255028a0. [DOI] [PubMed] [Google Scholar]
- Adler J., Wood H. A., Bozarth R. F. Virus-like particles from killer, neutral, and sensitive strains of Saccharomyces cerevisiae. J Virol. 1976 Feb;17(2):472–476. doi: 10.1128/jvi.17.2.472-476.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bevan E. A., Herring A. J., Mitchell D. J. Preliminary characterization of two species of dsRNA in yeast and their relationship to the "killer" character. Nature. 1973 Sep 14;245(5420):81–86. doi: 10.1038/245081b0. [DOI] [PubMed] [Google Scholar]
- Both G. W., Furuichi Y., Muthukrishnan S., Shatkin A. J. Ribosome binding to reovirus mRNA in protein synthesis requires 5' terminal 7-methylguanosine. Cell. 1975 Oct;6(2):185–195. doi: 10.1016/0092-8674(75)90009-4. [DOI] [PubMed] [Google Scholar]
- Buck K. W. Replication of double-stranded RNA in particles of Penicillium stoloniferum virus S. Nucleic Acids Res. 1975 Oct;2(10):1889–1902. doi: 10.1093/nar/2.10.1889. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bussey H. Effects of yeast killer factor on sensitive cells. Nat New Biol. 1972 Jan 19;235(55):73–75. doi: 10.1038/newbio235073a0. [DOI] [PubMed] [Google Scholar]
- Chow N. L., Shatkin A. J. Blocked and unblocked 5' termini in reovirus genome RNA. J Virol. 1975 May;15(5):1057–1064. doi: 10.1128/jvi.15.5.1057-1064.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dahlberg J. E. Terminal sequences of bacteriophage RNAs. Nature. 1968 Nov 9;220(5167):548–552. doi: 10.1038/220548a0. [DOI] [PubMed] [Google Scholar]
- Herring A. J., Bevan E. A. Virus-like particles associated with the double-stranded RNA species found in killer and sensitive strains of the yeast Saccharomyces cerevisiae. J Gen Virol. 1974 Mar;22(3):387–394. doi: 10.1099/0022-1317-22-3-387. [DOI] [PubMed] [Google Scholar]
- Hewlett M. J., Rose J. K., Baltimore D. 5'-terminal structure of poliovirus polyribosomal RNA is pUp. Proc Natl Acad Sci U S A. 1976 Feb;73(2):327–330. doi: 10.1073/pnas.73.2.327. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hickey E. D., Weber L. A., Baglioni C. Inhibition of initiation of protein synthesis by 7-methylguanosine-5'-monophosphate. Proc Natl Acad Sci U S A. 1976 Jan;73(1):19–23. doi: 10.1073/pnas.73.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lemke P. A., Nash C. H. Fungal viruses. Bacteriol Rev. 1974 Mar;38(1):29–56. doi: 10.1128/br.38.1.29-56.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roblin R. The 5'-terminus of bacteriophage R17 RNA: pppGp. J Mol Biol. 1968 Jan 14;31(1):51–61. doi: 10.1016/0022-2836(68)90053-3. [DOI] [PubMed] [Google Scholar]
- Rubin G. M. The nucleotide sequence of Saccharomyces cerevisiae 5.8 S ribosomal ribonucleic acid. J Biol Chem. 1973 Jun 10;248(11):3860–3875. [PubMed] [Google Scholar]
- Schonberg M., Silverstein S. C., Levin D. H., Acs G. Asynchronous synthesis of the complementary strands of the reovirus genome. Proc Natl Acad Sci U S A. 1971 Feb;68(2):505–508. doi: 10.1073/pnas.68.2.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shatkin A. J., Both G. W. Reovirus mRNA: transcription and translation. Cell. 1976 Mar;7(3):305–313. doi: 10.1016/0092-8674(76)90159-8. [DOI] [PubMed] [Google Scholar]
- Vodkin M., Katterman F., Fink G. R. Yeast killer mutants with altered double-stranded ribonucleic acid. J Bacteriol. 1974 Feb;117(2):681–686. doi: 10.1128/jb.117.2.681-686.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Woods D. R., Bevan E. A. Studies on the nature of the killer factor produced by Saccharomyces cerevisiae. J Gen Microbiol. 1968 Apr;51(1):115–126. doi: 10.1099/00221287-51-1-115. [DOI] [PubMed] [Google Scholar]
- Young R. A., Perlman P. S. "Killer" character does not influence the transmission of mitochondrial genes in Saccharomyces cerevisiae. J Bacteriol. 1975 Oct;124(1):290–295. doi: 10.1128/jb.124.1.290-295.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zimmern D. The 5' end group of tobacco mosaic virus RNA is m7G5' ppp5' Gp. Nucleic Acids Res. 1975 Jul;2(7):1189–1201. doi: 10.1093/nar/2.7.1189. [DOI] [PMC free article] [PubMed] [Google Scholar]
- al-Aidroos K., Somers J. M., Bussey H. Retention of cytoplasmic killer determinants in yeast cells after removal of mitochondrial DNA by ethidium bromide. Mol Gen Genet. 1973 May 28;122(4):323–330. doi: 10.1007/BF00269432. [DOI] [PubMed] [Google Scholar]