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. 1976 Oct;3(10):2645–2663. doi: 10.1093/nar/3.10.2645

Isolation of native DNA fragments containing structural genes at the beginning, in the middle or at the end of the coding strand.

G N Yenikolopov, A P Ryskov, T Nitta, G P Georgiev
PMCID: PMC343119  PMID: 995647

Abstract

A method for the isolation of the DNA fragments containing structural genes at the beginning, in the middle and at the end of the coding strand is described. It involves mechanical or enzymatic shearing, exonuclease treatment, hybridization with mRNA and subsequent retention of DNA-mRNA complex on poly(U)-Sepharose. Hybridization of the DNA fragments with mRNA takes place with high specificity and isolated material is enriched in structural genes. Applications of this method for gene isolation are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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