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. 1976 Oct;3(10):2771–2787. doi: 10.1093/nar/3.10.2771

Absence of 5' terminal capping in encephalomyocarditis virus RNA.

D Frisby, M Eaton, P Fellner
PMCID: PMC343127  PMID: 186763

Abstract

The nature of the 5' terminus of encephalomyocarditis (EMC) virion RNA has been investigated. We have failed to detect any capped products or nucleoside polyphosphates arising upon complete digestion of the RNA with T1, T2, and pancreatic ribonucleases, and it would therefore appear that the 5' terminus of EMC virus RNA is not phosphorylated and not capped with m7G. EMC virions do contain, however, large amounts of all four 5'-monosubstituted nucleoside triphosphates (4.2M pppG; 16.4M pppA; 3.OM pppU and 2.5M pppC), of which at least a proportion (about 15-20%) appear to remain bound to fully denatured RNA in the presence of divalent cations.

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