. Author manuscript; available in PMC: 2013 Jun 12.

Published in final edited form as: Biochemistry. 2012 May 25;51(23):4713–4722. doi: 10.1021/bi300037k

Table 2.

Hymeglusin Inhibition of E. FAECALIS and Human HMG-CoA synthase^a

Sample/Enzyme	Time to growth curve inflection point (hours)	Inactivation Kinetic Parameters	Rate of activity restoration by 1 mM hydroxylamine (mUnits activity recovered/minute)
E. faecalis cells	0.71±0.03
E. faecalis cells +hymeglusin	3.05±0.04
E. faecalis mvaS		Nonlinear fit method:	0.0012±0.0002
		K_I = 700±18.5 nM
		k_inact = 3.5±0.6 min⁻¹
		k_inact/K_I= 0.0050±0.0016 nM⁻¹min⁻¹
		Graphical method:
		K_I = 606 nM
		k_inact = 2.75 min⁻¹
		k_inact/K_I=0.0045 nM⁻¹min⁻¹
Human HMGCS^b		K_I = 53.7 nM	0.0097±0.0001
		k_inact = 1.06 min⁻¹
		k_inact/K_I = 0.0197 nM⁻¹min⁻¹

Experimental details are provided in the legends to Figure 1, Figure 2, and the Experimental Procedures section.

Values reported by Roskosz et al. (8).