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. 1976 Nov;3(11):2985–2999. doi: 10.1093/nar/3.11.2985

Sheared DNA fragment sizing: comparison of techniques.

C P Ordahl, T R Johnson, A I Caplan
PMCID: PMC343146  PMID: 1034292

Abstract

DNA fragmented by conventional French press shearing procedures (30,000 lbs/in2) has a number-average fragment size of 230 base pairs. This is considerably smaller than the 450 base pairs typically reported for DNA sheared by this method. Comparison of 5 sizing techniques indicates that sheared DNA fragment size is overestimated by either measurement of velocity sedimentation or Kleinschmidt Electron Microscopic visualization. Both adsorption grid electron microscopic visualization and gel electrophoresis yield the most reliable estimates of the mean size of small DNA fragment populations. In addition, the assessment of fragment size distribution (not possible from sedimentation analysis) potentially allows more critical evaluation of DNA hybridization and reassociation kinetic and measurement parameters.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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