Abstract
The incorporation of 3H-UTP into RNA by isolated rat bone marrow nuclei is stimulated by testosterone. This effect is hormone and tissue specific. Using alpha-amanitine and different ionic strength conditions it was found that testosterone enhances preferentially RNA polymerase I activity. The sedimentation pattern of RNA isolated from bone marrow nuclei shows that the synthesis of RNA species within the 14-30 S range is mainly stimulated by the hormone.
Full text
PDF









Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Hardin J. W., Clark J. H., Glasser S. R., Peck E. J., Jr RNA polymerase activity and uterine growth: Differential stimulation by estradiol, estriol, and nafoxidine. Biochemistry. 1976 Apr 6;15(7):1370–1374. doi: 10.1021/bi00652a003. [DOI] [PubMed] [Google Scholar]
- Jaehning J. A., Stewart C. C., Roeder R. G. DNA-dependent RNA polymerase levels during the response of human peripheral lymphocytes to phytohemagglutinin. Cell. 1975 Jan;4(1):51–57. doi: 10.1016/0092-8674(75)90133-6. [DOI] [PubMed] [Google Scholar]
- McEwen C. R. Tables for estimating sedimentation through linear concentration gradients of sucrose solution. Anal Biochem. 1967 Jul;20(1):114–149. doi: 10.1016/0003-2697(67)90271-0. [DOI] [PubMed] [Google Scholar]
- Milin B., Roy A. K. Androgen "receptor" in rat liver: cytosol "receptor" deficiency in pseudohermaphrodite male rats. Nat New Biol. 1973 Apr 25;242(121):248–250. doi: 10.1038/newbio242248a0. [DOI] [PubMed] [Google Scholar]
- Minguell J. J., Sierralta W. D. Molecular mechanism of action of the male sex hormones. J Endocrinol. 1975 May;65(2):287–315. doi: 10.1677/joe.0.0650287. [DOI] [PubMed] [Google Scholar]
- Minguell J., Garavagno A., Yanez J. Effect of methyltestosterone on the nucleic acid metabolism of rat bone marrow cells. Proc Soc Exp Biol Med. 1971 Nov;138(2):438–440. doi: 10.3181/00379727-138-35914. [DOI] [PubMed] [Google Scholar]
- Nicol A. G., Conkie D., Lanyon W. G., Drewienkiewicz C. E., Williamson R., Paul J. Characteristics of erythropoietin-induced RNA from foetal mouse liver erythropoietic cell cultures and the effects of 5-fluorodeoxyuridine. Biochim Biophys Acta. 1972 Aug 25;277(2):342–353. doi: 10.1016/0005-2787(72)90416-9. [DOI] [PubMed] [Google Scholar]
- Sierralta W., Minguell J. Ribonuclease activity in rat bone marrow nuclei. Biochem Biophys Res Commun. 1970 Oct 9;41(1):50–56. doi: 10.1016/0006-291x(70)90467-5. [DOI] [PubMed] [Google Scholar]
- Valladares L., Minguell J. Characterization of a nuclear receptor for testosterone in rat bone marrow. Steroids. 1975 Jan;25(1):13–21. doi: 10.1016/s0039-128x(75)80003-1. [DOI] [PubMed] [Google Scholar]
- Weinmann R., Roeder R. G. Role of DNA-dependent RNA polymerase 3 in the transcription of the tRNA and 5S RNA genes. Proc Natl Acad Sci U S A. 1974 May;71(5):1790–1794. doi: 10.1073/pnas.71.5.1790. [DOI] [PMC free article] [PubMed] [Google Scholar]
