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. 1976 Dec;3(12):3337–3347. doi: 10.1093/nar/3.12.3337

Recognition of polynucleotides by antibodies to poly(I), poly(C).

M Guigues, M Leng
PMCID: PMC343178  PMID: 1005120

Abstract

The binding of anti poly(I). poly (C) Fab fragments to double or triple stranded polynucletides has been studied by fluorescence. Association constants were deduced from competition experiments. The comparison of the association constants leads to the conclusion that several atoms of the base residues do not interact with the amino acid residues of the binding site of Fab fragment while the hydroxyl groups of furanose rings interact. These results suggest that the Fab fragments do not bind to the major groove of the double stranded polynucleotides. An interaction between the C(2)O group of pyrimidine residues and Fab fragments cannot be excluded. Circular dichroism of poly(I). poly(C) or poly(I). poly(br5C)-Fab fragments complexes are very different from the circular dichroism of free polynucleotides which suggests a deformation of the polynucleotides bound to the Fab fragments.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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