Skip to main content
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1976 Dec;3(12):3359–3367. doi: 10.1093/nar/3.12.3359

End group of naturally terminated and UV lesion terminated T7 in vitro RNA.

R Ali, R Millette, W Sauerbier
PMCID: PMC343180  PMID: 1005122

Abstract

The 3' terminal nucleosides of RNA transcribed in vitro by E. coli RNA polymerase from T7 DNA and UV irradiated TN DNA were determined. The 3' terminal nucleoside of naturally terminated (t1 termination site) RNA cytidine. In the case of RNA terminated at UV lesions, it is cytidine in 0 per cent of the molecules and adenosine in the remaining 30 per cent. Cytidine trialcohols are labile in high concentrations of KOH and at high temperature and appear to convert to uridine.

Full text

PDF
3359

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bräutigam A. R., Sauerbier W. Transcription unit mapping in bacteriophage T7. II. Proportionality of number of gene copies, mRNA, and gene product. J Virol. 1974 May;13(5):1110–1117. doi: 10.1128/jvi.13.5.1110-1117.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Hackett P. B., Sauerbier W. Radiological mapping of the ribosomal RNA transcription unit in E. coli. Nature. 1974 Oct 18;251(5476):639–641. doi: 10.1038/251639a0. [DOI] [PubMed] [Google Scholar]
  3. Hackett P. B., Sauerbier W. The transcriptional organization of the ribosomal RNA genes in mouse L cells. J Mol Biol. 1975 Jan 25;91(3):235–256. doi: 10.1016/0022-2836(75)90378-2. [DOI] [PubMed] [Google Scholar]
  4. Lebowitz P., Weissman S. M., Radding C. M. Nucleotide sequence of a ribonucleic acid transcribed in vitro from lambda phage deoxyribonucleic acid. J Biol Chem. 1971 Aug 25;246(16):5120–5139. [PubMed] [Google Scholar]
  5. Loening U. E. The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem J. 1967 Jan;102(1):251–257. doi: 10.1042/bj1020251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Michalke H., Bremer H. RNA synthesis in Escherichia coli after irradiation with ultraviolet light. J Mol Biol. 1969 Apr 14;41(1):1–23. doi: 10.1016/0022-2836(69)90122-3. [DOI] [PubMed] [Google Scholar]
  7. Millette R. L., Trotter C. D. Initiation and release of RNA by DNA-dependent RNA polymerase. Proc Natl Acad Sci U S A. 1970 Jul;66(3):701–708. doi: 10.1073/pnas.66.3.701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Peters G. G., Hayward R. S. Transcriptional termination in vitro: the 3'-terminal sequence of coliphage T7 "early" RNA. Biochem Biophys Res Commun. 1974 Nov 27;61(2):809–816. doi: 10.1016/0006-291x(74)91029-8. [DOI] [PubMed] [Google Scholar]
  9. SAUERBIER W. ROLE OF CYTOSINE PHOTOPRODUCTS IN ULTRAVIOLET INACTIVATION OF BACTERIOPHAGE T1. J Mol Biol. 1964 Dec;10:551–553. doi: 10.1016/s0022-2836(64)80077-2. [DOI] [PubMed] [Google Scholar]
  10. Sauerbier W., Bräutigam A. R. A simple method for isolating RNA from bacteria. Biochim Biophys Acta. 1970 Jan 21;199(1):36–40. doi: 10.1016/0005-2787(70)90692-1. [DOI] [PubMed] [Google Scholar]
  11. Sauerbier W., Millette R. L., Hackett P. B., Jr The effects of ultraviolet irradiation on the transcription of T4 DNA. Biochim Biophys Acta. 1970;209(2):368–386. doi: 10.1016/0005-2787(70)90735-5. [DOI] [PubMed] [Google Scholar]
  12. Setlow J. K., Boling M. E., Bollum F. J. The chemical nature of photoreactivable lesions in DNA. Proc Natl Acad Sci U S A. 1965 Jun;53(6):1430–1436. doi: 10.1073/pnas.53.6.1430. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Steinschneider A., Fraenkel-Conrat H. Studies of nucleotide sequences in tobacco mosaic virus ribonucleic acid. IV. Use of aniline in stepwise degradation. Biochemistry. 1966 Aug;5(8):2735–2743. doi: 10.1021/bi00872a034. [DOI] [PubMed] [Google Scholar]
  14. Studier F. W. Bacteriophage T7. Science. 1972 Apr 28;176(4033):367–376. doi: 10.1126/science.176.4033.367. [DOI] [PubMed] [Google Scholar]
  15. WULFF D. L. THE ROLE OF THYMINE DIMER IN THE PHOTO-INACTIVATION OF THE BACTERIOPHAGE T4V1. J Mol Biol. 1963 Oct;7:431–441. doi: 10.1016/s0022-2836(63)80035-2. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES