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. 1976 Dec;3(12):3397–3408. doi: 10.1093/nar/3.12.3397

Nucleoside-3'-phosphotriesters as key intermediates for the oligoribonucleotide synthesis. III. An improved preparation of nucleoside 3'-phosphotriesters, their 1H NMR characterization and new conditions for removal of 2-cyanoethyl group.

R W Adamiak, M Z Barciszewska, E Biala, K Grzéskowiak, R Kierzek, A Kraszewski, W T Markiewicz, M Wiewiórowski
PMCID: PMC343184  PMID: 1005124

Abstract

An improved procedure for the transformation of 5'-O-monomethoxytrityl-2'-O-acetyl-3'-phosphates of uridine la, inosine ib and 6-N-benzoyladenosine lc into corresponding 3'/2,2,2-trichloroethyl, 2-cyanoethyl/-phosphates iiaic is reported. H NMR characterization of nucleoside 3'-phosphotriesters is presented. New conditions i.e. anhydrous triethylamine-pyridine treatment have been found for the selective removal of 2-cyanoethyl group from nucleoside 3'-phosphotriesters in the presence of neighbouring 2'-O-acetyl one.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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