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. 1977 Nov;4(11):3753–3768. doi: 10.1093/nar/4.11.3753

Intramolecular base composition heterogeneity of human DNA.

D L Vizard, F P Rinehart, C M Rubin, C W Schmid
PMCID: PMC343198  PMID: 593884

Abstract

The intramolecular base composition heterogeneity of human DNA has been investigated by electron microscopic observations of partially denatured structures and by equilibrium solution thermal denaturation techniques. DNA sequences having an average length of less than 2000 base pairs are found to be heterogeneous in base composition. These heterogeneous sequences occupy a minimum of 67 to 81% of the human genome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ansevin A. T., Vizard D. L., Brown B. W., McConathy J. High-resolution thermal denaturation of DNA. I. Theoretical and practical considerations for the resolution of thermal subtransitions. Biopolymers. 1976 Jan;15(1):153–174. doi: 10.1002/bip.1976.360150111. [DOI] [PubMed] [Google Scholar]
  2. Crain W. R., Davidson E. H., Britten R. J. Contrasting patterns of DNA sequence arrangement in Apis mellifera (honeybee) and Musca domestica (housefly). Chromosoma. 1976 Dec 6;59(1):1–12. doi: 10.1007/BF00327705. [DOI] [PubMed] [Google Scholar]
  3. Davidson E. H., Galau G. A., Angerer R. C., Britten R. J. Comparative aspects of DNA organization in Metazoa. Chromosoma. 1975 Jul 21;51(3):253–259. doi: 10.1007/BF00284818. [DOI] [PubMed] [Google Scholar]
  4. Davis R. W., Hyman R. W. A study in evolution: the DNA base sequence homology between coliphages T7 and T3. J Mol Biol. 1971 Dec 14;62(2):287–301. doi: 10.1016/0022-2836(71)90428-1. [DOI] [PubMed] [Google Scholar]
  5. Deininger P. L., Schmid C. W. An electron microscope study of the DNA sequence organization of the human genome. J Mol Biol. 1976 Sep 25;106(3):773–790. doi: 10.1016/0022-2836(76)90264-3. [DOI] [PubMed] [Google Scholar]
  6. Filipski J., Thiery J. P., Bernardi G. An analysis of the bovine genome by Cs2SO4-Ag density gradient centrifugation. J Mol Biol. 1973 Oct 15;80(1):177–197. doi: 10.1016/0022-2836(73)90240-4. [DOI] [PubMed] [Google Scholar]
  7. GEIDUSCHEK E. P. On the factors controlling the reversibility of DNA denaturation. J Mol Biol. 1962 Jun;4:467–487. doi: 10.1016/s0022-2836(62)80103-x. [DOI] [PubMed] [Google Scholar]
  8. Lyubchenko Y. L., Frank-Kamenetskii M. D., Vologodskii A. V., Lazurkin Y. S., Gause G. G., Jr Fine structure of DNA melting curves. Biopolymers. 1976 Jun;15(6):1019–1036. doi: 10.1002/bip.1976.360150602. [DOI] [PubMed] [Google Scholar]
  9. Macaya G., Thiery J. P., Bernardi G. An approach to the organization of eukaryotic genomes at a macromolecular level. J Mol Biol. 1976 Nov;108(1):237–254. doi: 10.1016/s0022-2836(76)80105-2. [DOI] [PubMed] [Google Scholar]
  10. Manning J. E., Schmid C. W., Davidson N. Interspersion of repetitive and nonrepetitive DNA sequences in the Drosophila melanogaster genome. Cell. 1975 Feb;4(2):141–155. doi: 10.1016/0092-8674(75)90121-x. [DOI] [PubMed] [Google Scholar]
  11. Saunders G. F., Shirakawa S., Saunders P. P., Arrighi F. E., Hsu T. C. Populations of repeated DNA sequences in the human genome. J Mol Biol. 1972 Feb 14;63(3):323–334. doi: 10.1016/0022-2836(72)90430-5. [DOI] [PubMed] [Google Scholar]
  12. Schmid C. W., Deininger P. L. Sequence organization of the human genome. Cell. 1975 Nov;6(3):345–358. doi: 10.1016/0092-8674(75)90184-1. [DOI] [PubMed] [Google Scholar]
  13. Schmid C. W., Hearst J. E. Sedimentation equilibrium of DNA samples heterogeneous in density. Biopolymers. 1972;11(9):1913–1918. doi: 10.1002/bip.1972.360110911. [DOI] [PubMed] [Google Scholar]
  14. Sueoka N. A STATISTICAL ANALYSIS OF DEOXYRIBONUCLEIC ACID DISTRIBUTION IN DENSITY GRADIENT CENTRIFUGATION. Proc Natl Acad Sci U S A. 1959 Oct;45(10):1480–1490. doi: 10.1073/pnas.45.10.1480. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Thiery J. P., Macaya G., Bernardi G. An analysis of eukaryotic genomes by density gradient centrifugation. J Mol Biol. 1976 Nov;108(1):219–235. doi: 10.1016/s0022-2836(76)80104-0. [DOI] [PubMed] [Google Scholar]
  16. Vizard D. L., Ansevin A. T. High resolution thermal denaturation of DNA: thermalites of bacteriophage DNA. Biochemistry. 1976 Feb 24;15(4):741–750. doi: 10.1021/bi00649a004. [DOI] [PubMed] [Google Scholar]
  17. Wolfson J., Dressler D., Magazin M. Bacteriophage T7 DNA replication: a linear replicating intermediate (gradient centrifugation-electron microscopy-E. coli-DNA partial denaturation). Proc Natl Acad Sci U S A. 1972 Feb;69(2):499–504. doi: 10.1073/pnas.69.2.499. [DOI] [PMC free article] [PubMed] [Google Scholar]

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