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. 1977 Nov;4(11):3887–3900. doi: 10.1093/nar/4.11.3887

Purification and properties of tyrosyl tRNA synthetase of rat liver.

Y S Prasada Rao, P R Srinivasan
PMCID: PMC343208  PMID: 593893

Abstract

Purification of rat liver tyrosine tRNA synthetase yields two protein fractions A and B and both fractions are required for charging of tyrosine to tRNAtyr. Fraction B catalyzes the activation of tyrosine. Fractions A and B have been purified to near homogeneity and they are composed of single polypeptide chains of 62,000 daltons each. Gel filtration studies suggest a molecular weight of 120,000 for the synthetase.

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Selected References

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