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. 1977 Nov;4(11):4053–4062. doi: 10.1093/nar/4.11.4053

Specific fragmentation of T7 phage DNA at low-melting sites.

V M Pavlov, Y L Lyubchenko, A S Borovik, Y S Lazurkin
PMCID: PMC343221  PMID: 593901

Abstract

A method has been developed for selective fragmentation of T7 DNA at AT-rich regions. The molecules have been subjected to complete digestion with single-strand-specific SI endonuclease after fixation of DNA AT-rich regions in the denatured state by glyoxal. The treatment resulted in three fragments having molecular weights of 13.6 +/- 0.4, 8.2 +/- 0.4 and 3.5 +/- 0.16 megadaltons as determined by electron microscopy. The position of these fragments along the T7 DNA molecule has been determined by means of analysis of the intermediates during SI-cleavage.

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Selected References

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