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. 1977 Dec;4(12):4371–4389. doi: 10.1093/nar/4.12.4371

The nucleotide sequence at the termini of adenovirus type 5 DNA.

P H Steenbergh, J Maat, H van Ormondt, J S Sussenbach
PMCID: PMC343247  PMID: 600799

Abstract

The sequences of the first 194 base pairs at both termini of adenovirus type 5 (Ad5) DNA have been determined, using the chemical degradation technique developed by Maxam and Gilbert (Proc. Nat. Acad. Sci. USA 74 (1977), pp. 560-564). The nucleotide sequences 1-75 were confirmed by analysis of labeled RNA transcribed from the terminal HhaI fragments in vitro. The sequence data show that Ad5 DNA has a perfect inverted terminal repetition of 103 base pairs long.

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Selected References

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  1. Ariga H., Shimojo H. Initiation and termination sites of adenovirus 12 DNA replication. Virology. 1977 May 15;78(2):415–424. doi: 10.1016/0042-6822(77)90118-0. [DOI] [PubMed] [Google Scholar]
  2. Blackburn E. H. Transcription by Escherichia coli RNA polymerase of a single-stranded fragment by bacteriophage phiX174 DNA 48 residues in length. J Mol Biol. 1975 Apr 15;93(3):367–374. doi: 10.1016/0022-2836(75)90283-1. [DOI] [PubMed] [Google Scholar]
  3. Brownlee G. G., Sanger F. Chromatography of 32P-labelled oligonucleotides on thin layers of DEAE-cellulose. Eur J Biochem. 1969 Dec;11(2):395–399. doi: 10.1111/j.1432-1033.1969.tb00786.x. [DOI] [PubMed] [Google Scholar]
  4. Carusi E. A. Evidence for blocked 5'-termini in human adenovirus DNA. Virology. 1977 Jan;76(1):380–394. doi: 10.1016/0042-6822(77)90310-5. [DOI] [PubMed] [Google Scholar]
  5. Cavalier-Smith T. Palindromic base sequences and replication of eukaryote chromosome ends. Nature. 1974 Aug 9;250(5466):467–470. doi: 10.1038/250467a0. [DOI] [PubMed] [Google Scholar]
  6. Flint S. J., Berget S. M., Sharp P. A. Characterization of single-stranded viral DNA sequences present during replication of adenovirus types 2 and 5. Cell. 1976 Dec;9(4 Pt 1):559–571. doi: 10.1016/0092-8674(76)90038-6. [DOI] [PubMed] [Google Scholar]
  7. Galibert F., Sedat J., Ziff E. Direct determination of DNA nucleotide sequences: structure of a fragment of bacteriophage phiX172 DNA. J Mol Biol. 1974 Aug 15;87(3):377–407. doi: 10.1016/0022-2836(74)90093-x. [DOI] [PubMed] [Google Scholar]
  8. Garon C. F., Berry K. W., Rose J. A. A unique form of terminal redundancy in adenovirus DNA molecules. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2391–2395. doi: 10.1073/pnas.69.9.2391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Green M., Piña M., Kimes R., Wensink P. C., MacHattie L. A., Thomas C. A., Jr Adenovirus DNA. I. Molecular weight and conformation. Proc Natl Acad Sci U S A. 1967 May;57(5):1302–1309. doi: 10.1073/pnas.57.5.1302. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Horwitz M. S. Bidirectional replication of adenovirus type 2 DNA. J Virol. 1976 Apr;18(1):307–315. doi: 10.1128/jvi.18.1.307-315.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lavelle G., Patch C., Khoury G., Rose J. Isolation and partial characterization of single-stranded adenoviral DNA produced during synthesis of adenovirus type 2 DNA. J Virol. 1975 Oct;16(4):775–782. doi: 10.1128/jvi.16.4.775-782.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Levine A. J., van der Vliet P. C., Sussenbach J. S. The replication of papovavirus and adenovirus DNA. Curr Top Microbiol Immunol. 1976;73:67–124. doi: 10.1007/978-3-642-66306-2_3. [DOI] [PubMed] [Google Scholar]
  13. Mansfeld A. D., Vereijken J. M., Jansz H. S. The nucleotide sequence of a DNA fragment, 71 base pairs in length, near the origin of DNA replication of bacteriophage 0X174. Nucleic Acids Res. 1976 Oct;3(10):2827–2844. doi: 10.1093/nar/3.10.2827. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mulder C., Arrand J. R., Delius H., Keller W., Pettersson U., Roberts R. J., Sharp P. A. Cleavage maps of DNA from adenovirus types 2 and 5 by restriction endonucleases EcoRI and HpaI. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):397–400. doi: 10.1101/sqb.1974.039.01.051. [DOI] [PubMed] [Google Scholar]
  16. Padmanabhan R., Padmanabhan R., Green M. Evidence for palindromic sequences near the termini of adenovirus 2 DNA. Biochem Biophys Res Commun. 1976 Apr 19;69(4):860–867. doi: 10.1016/0006-291x(76)90453-8. [DOI] [PubMed] [Google Scholar]
  17. Rekosh D. M., Russell W. C., Bellet A. J., Robinson A. J. Identification of a protein linked to the ends of adenovirus DNA. Cell. 1977 Jun;11(2):283–295. doi: 10.1016/0092-8674(77)90045-9. [DOI] [PubMed] [Google Scholar]
  18. Richardson C. C. Phosphorylation of nucleic acid by an enzyme from T4 bacteriophage-infected Escherichia coli. Proc Natl Acad Sci U S A. 1965 Jul;54(1):158–165. doi: 10.1073/pnas.54.1.158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Roberts R. J., Arrand J. R., Keller W. The length of the terminal repetition in adenovirus-2 DNA. Proc Natl Acad Sci U S A. 1974 Oct;71(10):3829–3833. doi: 10.1073/pnas.71.10.3829. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Robinson A. J., Bellett J. D. A circular DNA-protein complex adenoviruses and its possible role in DNA replication. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):523–531. doi: 10.1101/sqb.1974.039.01.064. [DOI] [PubMed] [Google Scholar]
  21. Sambrook J., Williams J., Sharp P. A., Grodzicker T. Physical mapping of temperature-sensitive mutations of adenoviruses. J Mol Biol. 1975 Sep 25;97(3):369–390. doi: 10.1016/s0022-2836(75)80046-5. [DOI] [PubMed] [Google Scholar]
  22. Sharp P. A., Sugden B., Sambrook J. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry. 1973 Jul 31;12(16):3055–3063. doi: 10.1021/bi00740a018. [DOI] [PubMed] [Google Scholar]
  23. Smith H. O., Birnstiel M. L. A simple method for DNA restriction site mapping. Nucleic Acids Res. 1976 Sep;3(9):2387–2398. doi: 10.1093/nar/3.9.2387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Southern E. M., Mitchell A. R. Chromatography of nucleic acid digests on thin layers of cellulose impregnated with polyethyleneimine. Biochem J. 1971 Jul;123(4):613–617. doi: 10.1042/bj1230613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Steenbergh P. H., Sussenbach J. S., Roberts R. J., Jansz H. S. The 3'-terminal nucleotide sequences of adenovirus types 2 and 5 DNA. J Virol. 1975 Feb;15(2):268–272. doi: 10.1128/jvi.15.2.268-272.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Sussenbach J. S., Kuijk M. G. Studies on the mechanism of replication of adenovirus DNA. V. The location of termini of replication. Virology. 1977 Mar;77(1):149–157. doi: 10.1016/0042-6822(77)90414-7. [DOI] [PubMed] [Google Scholar]
  27. Sussenbach J. S. On the fate of adenovirus DNA in KB cells. Virology. 1971 Dec;46(3):969–972. doi: 10.1016/0042-6822(71)90099-7. [DOI] [PubMed] [Google Scholar]
  28. Sussenbach J. S., Tolun A., Pettersson U. Nature of the single-stranded DNA in replicating adenovirus type 5 DNA. J Virol. 1976 Nov;20(2):532–534. doi: 10.1128/jvi.20.2.532-534.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Volckaert G., Jou W. M., Fiers W. Analysis of 32P-labeled bacteriophage MS2 RNA by a mini-fingerprinting procedure. Anal Biochem. 1976 May 7;72:433–446. doi: 10.1016/0003-2697(76)90551-0. [DOI] [PubMed] [Google Scholar]
  30. Weingärtner B., Winnacker E. L., Tolun A., Pettersson U. Two complementary strand-specific termination sites for adenovirus DNA replication. Cell. 1976 Oct;9(2):259–268. doi: 10.1016/0092-8674(76)90117-3. [DOI] [PubMed] [Google Scholar]
  31. Wolfson J., Dressler D. Adenovirus-2 DNA contains an inverted terminal repetition. Proc Natl Acad Sci U S A. 1972 Oct;69(10):3054–3057. doi: 10.1073/pnas.69.10.3054. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Wu M., Roberts R. J., Davidson N. Structure of the inverted terminal repetition of adenovirus type 2 DNA. J Virol. 1977 Feb;21(2):766–777. doi: 10.1128/jvi.21.2.766-777.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. van der Eb A. J., van Kesteren L. W., van Bruggen E. F. Structural properties of adenovirus DNA's. Biochim Biophys Acta. 1969 Jun 17;182(2):530–541. doi: 10.1016/0005-2787(69)90205-6. [DOI] [PubMed] [Google Scholar]

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