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. 1977 Dec;4(12):4411–4424. doi: 10.1093/nar/4.12.4411

Isolation and partial characterization of poly (A)-containing 7.5S messenger RNA from rat liver mitochondria.

O I Kisselev, V S Gaitskhoki, S A Neifakh
PMCID: PMC343249  PMID: 600801

Abstract

Poly(A)-containing low molecular weight (7.5S) messenger RNA was isolated in a highly purified form from both polyribosomes and post-polysomal supernatant of rat liver mitochondria. Both mRNA's contain rather short poly(A) tracts (40-70 mononucleotides) according to a profile of their elution from poly(U)-Sepharose column with a gradient of formamide concentration. Both mRNA's when added to a preincubated mitochondrial lysate programmed the synthesis of a hydrophobic polypeptide of a molecular weight about 9000 daltons which was soluble in the neutral chloroform-methanol mixture.

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Selected References

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