Figure 1.

Effect of LOX inhibition on marrow fibrosis in vivo. (A) Representative hematoxylin and eosin (left column) and Gomori silver (right column) staining of longitudinal sections of femurs from wild-type and GATA-1^low (male littermates), control, or BAPN-treated mice (10.5 weeks old at the time of collection). Original magnifications: left column, ×400; and right column, ×600. Arrows indicate the large presence of MKs (hematoxylin and eosin stain) and the accumulation of reticulin fibers in the GATA-1^low mice. Images obtained using Nikon Eclipse 50i microscope equipped with a 40× Plan (numerical aperture 0.65) and a 60× Plan Fluor (numerical aperture 0.85) objectives. Digital images acquired using a SPOT 2 megapixel camera and SPOT 5.0 acquisition software. (B) Quantification of fibrosis in BAPN or vehicle-treated GATA-1^low mice. Fibers were measured in arbitrary units from stained sections. Data are represented as absolute values (top panel) or as mean percent change compared with values recorded from vehicle-treated GATA-1^low mice (bottom panel). The mean values were obtained from 5 mice per group. **P < .05. Reproduced from Eliades et al⁷⁵ with permission.