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. 1974 Jan;1(1):35–43. doi: 10.1093/nar/1.1.35

2′-O-Methyl ribothymidine: a component of rabbit liver lysine transfer RNA

HJ Gross *, M Simsek , M Raba *, K Limburg *, J Heckman , UL RajBhandary
PMCID: PMC343321  PMID: 10793657

Abstract

One of the lysine transfer RNAs of rabbit liver is shown to contain 2′-O-methyl ribothymidine in place of ribothymidine. This represents the first demonstration of the presence of 2′-O-methyl ribothymidine in a nucleic acid.

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Selected References

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  1. Björk G. R., Isaksson L. A. Isolation of mutants of Escherichia coli lac king 5-methyluracil in transfer ribonucleic acid or 1-methylguanine in ribosomal RNA. J Mol Biol. 1970 Jul 14;51(1):83–100. doi: 10.1016/0022-2836(70)90272-x. [DOI] [PubMed] [Google Scholar]
  2. Dirheimer G., Ebel J. P., Bonnet J., Gangloff J., Keith G., Krebs B., Kuntzel B., Roy A., Weissenbach J., Werner C. Structure primaire des tRN. Biochimie. 1972;54(2):127–144. doi: 10.1016/s0300-9084(72)80097-x. [DOI] [PubMed] [Google Scholar]
  3. Gillam I., Millward S., Blew D., von Tigerstrom M., Wimmer E., Tener G. M. The separation of soluble ribonucleic acids on benzoylated diethylaminoethylcellulose. Biochemistry. 1967 Oct;6(10):3043–3056. doi: 10.1021/bi00862a011. [DOI] [PubMed] [Google Scholar]
  4. Johnson L., Hayashi H., Söll D. Isolation and properties of a transfer ribonucleic acid deficient in ribothymidine. Biochemistry. 1970 Jul 7;9(14):2823–2831. doi: 10.1021/bi00816a011. [DOI] [PubMed] [Google Scholar]
  5. Keith G., Picaud F., Weissenbach J., Ebel J. P., Petrissant G., Dirheimer G. The primary structure of rabbit liver tRNA Phe and its comparison with known tRNA Phe sequences. FEBS Lett. 1973 May 1;31(3):345–347. doi: 10.1016/0014-5793(73)80138-3. [DOI] [PubMed] [Google Scholar]
  6. Kimura-Harada F., Saneyoshi M., Nishimura S. 5-methyl-2-thiouridine: A new sulfur-containing minor constituent from rat liver glutamic acid and lysine tRNAs. FEBS Lett. 1971 Apr 2;13(6):335–338. doi: 10.1016/0014-5793(71)80254-5. [DOI] [PubMed] [Google Scholar]
  7. Nichols J. L., Lane B. G. Characterization of N4,O2'-dimethylcytidine, a rare nucleoside constituent of Escherichia coli 16-S RNA. Biochim Biophys Acta. 1968 Oct 29;166(3):605–615. doi: 10.1016/0005-2787(68)90367-5. [DOI] [PubMed] [Google Scholar]
  8. Petrissant G. Evidence for the absence of the G-T-psi-C sequence from two mammalian initiator transfer RNAs. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1046–1049. doi: 10.1073/pnas.70.4.1046. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. RajBhandary U. L., Faulkner R. D., Stuart A. Studies on polynucleotides. LXXIX. Yeast phenylalanine transfer ribonucleic acid: products obtained by degradation with pancreatic ribonuclease. J Biol Chem. 1968 Feb 10;243(3):575–583. [PubMed] [Google Scholar]
  10. RajBhandary U. L., Stuart A., Chang S. H. Studies on polynucleotides. LXXX. Yeast phenylalanine transfer ribonucleic acid: products obtained by degradation with ribonuclease T1. J Biol Chem. 1968 Feb 10;243(3):584–591. [PubMed] [Google Scholar]
  11. Roberts R. J. Structures of two glycyl-tRNAs from Staphylococcus epidermidis. Nat New Biol. 1972 May 10;237(71):44–45. doi: 10.1038/newbio237044a0. [DOI] [PubMed] [Google Scholar]
  12. Rosenthal L. J., Zamecnik P. C. Minor base composition of "70S-associated" 4S RNA from avian myeloblastosis virus. Proc Natl Acad Sci U S A. 1973 Mar;70(3):865–869. doi: 10.1073/pnas.70.3.865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sanger F., Brownlee G. G., Barrell B. G. A two-dimensional fractionation procedure for radioactive nucleotides. J Mol Biol. 1965 Sep;13(2):373–398. doi: 10.1016/s0022-2836(65)80104-8. [DOI] [PubMed] [Google Scholar]
  14. Simsek M., RajBhandary U. L. The primary structure of yeast initiator transfer ribonucleic acid. Biochem Biophys Res Commun. 1972 Oct 17;49(2):508–515. doi: 10.1016/0006-291x(72)90440-8. [DOI] [PubMed] [Google Scholar]
  15. Simsek M., Ziegenmeyer J., Heckman J., Rajbhandary U. L. Absence of the sequence G-T-psi-C-G(A)- in several eukaryotic cytoplasmic initiator transfer RNAs. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1041–1045. doi: 10.1073/pnas.70.4.1041. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Yang S., Reinitz E. R., Gefter M. L. Role of modifications in tyrosine transfer RNA. II. Ribothymidylate-deficient tRNA. Arch Biochem Biophys. 1973 Jul;157(1):55–62. doi: 10.1016/0003-9861(73)90389-5. [DOI] [PubMed] [Google Scholar]

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