Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1974 Feb;1(2):245–255. doi: 10.1093/nar/1.2.245

Calf lens crystallin messenger RNA's contain polynucleotide sequences rich in adenylic acid

Gianni Piperno 1, Umberto Bertazzoni 1, Anton JM Berns 1, Hans Bloemendal 1
PMCID: PMC343343  PMID: 4472020

Abstract

Calf lens messenger fractions have been purified on poly(dT)-cellulose. The purified RNA's have been analyzed as nucleosides by a method which can be used on a micro-scale with highly reproduceble results. Both the 10S and 14S lens mRNA contain poly A sequences. The poly A content found in 14S mRNA cannot account for the excess nucleotides in this message.

Full text

PDF
245

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adesnik M., Darnell J. E. Biogenesis and characterization of histone messenger RNA in HeLa cells. J Mol Biol. 1972 Jun 28;67(3):397–406. doi: 10.1016/0022-2836(72)90458-5. [DOI] [PubMed] [Google Scholar]
  2. Astell C. R., Smith M. Synthesis and properties of oligonucleotide--cellulose columns. Biochemistry. 1972 Oct 24;11(22):4114–4120. doi: 10.1021/bi00772a014. [DOI] [PubMed] [Google Scholar]
  3. Berns A. J., Bloemendal H., Kaufman S. J., Verma I. M. Synthesis of DNA complementary to 14S calf lens crystallin messenger RNA by reverse transcriptase. Biochem Biophys Res Commun. 1973 Jun 8;52(3):1013–1019. doi: 10.1016/0006-291x(73)91038-3. [DOI] [PubMed] [Google Scholar]
  4. Berns A. J.M., De Abreu R. A., Van Kraaikamp M., Benedetti E. L., Bloemendal H. Synthesis of lens protein in vitro V. Isolation of messenger-like RNA from lens by high resolution zonal centrifugation. FEBS Lett. 1971 Oct 15;18(1):159–163. doi: 10.1016/0014-5793(71)80434-9. [DOI] [PubMed] [Google Scholar]
  5. Berns A. J., Strous G. J., Bloemendal H. Heterologous in vitro synthesis of lens -crystallin polypeptide. Nat New Biol. 1972 Mar 1;236(61):7–9. doi: 10.1038/newbio236007a0. [DOI] [PubMed] [Google Scholar]
  6. Berns J. M., Schreurs V. V., van Kraaikamp M. W., Bloemendal H. Synthesis of lens protein in vitro. Translation of calf-lens messengers in heterologous systems. Eur J Biochem. 1973 Mar 15;33(3):551–557. doi: 10.1111/j.1432-1033.1973.tb02715.x. [DOI] [PubMed] [Google Scholar]
  7. Bertazzoni U., Campagnari F., De Luca U. A convenient solid-state substrate for assays of polynucleotide ligase: the poly(dA)-(poly(( 3 H)dT)-poly(dT))-cellulose. Biochim Biophys Acta. 1971 Jul 29;240(4):515–521. doi: 10.1016/0005-2787(71)90708-8. [DOI] [PubMed] [Google Scholar]
  8. Chen J. H., Lavers G. C., Spector A. Synthesis of complementary DNA from lens mRNA with RNA-dependent DNA polymerase. Biochem Biophys Res Commun. 1973 Jun 8;52(3):767–773. doi: 10.1016/0006-291x(73)91003-6. [DOI] [PubMed] [Google Scholar]
  9. Ehrlich S. D., Thiery J. P., Bernardi G. Analysis of nucleosides on columns of fractionated sephadex G-10. Biochim Biophys Acta. 1971 Aug 26;246(2):161–168. doi: 10.1016/0005-2787(71)90123-7. [DOI] [PubMed] [Google Scholar]
  10. Filipski J., Thiery J. P., Bernardi G. An analysis of the bovine genome by Cs2SO4-Ag density gradient centrifugation. J Mol Biol. 1973 Oct 15;80(1):177–197. doi: 10.1016/0022-2836(73)90240-4. [DOI] [PubMed] [Google Scholar]
  11. Mathews M. B., Osborn M., Berns A. J., Bloemendal H. Translation of two messenger RNAs from lens in a cell free system from Krebs II ascites cells. Nat New Biol. 1972 Mar 1;236(61):5–7. doi: 10.1038/newbio236005a0. [DOI] [PubMed] [Google Scholar]
  12. Pemberton R. E., Baglioni C. Duck hemoglobin messenger RNA contains a polynucleotide sequence rich in adenylic acid. J Mol Biol. 1972 Apr 14;65(3):531–535. doi: 10.1016/0022-2836(72)90207-0. [DOI] [PubMed] [Google Scholar]
  13. Piperno G., Bernardi G. Separation of nucleosides on polyacrylamide gel columns: further developments. Biochim Biophys Acta. 1971 May 27;238(3):388–396. doi: 10.1016/0005-2787(71)90613-7. [DOI] [PubMed] [Google Scholar]
  14. Thiery J. P., Ehrlich S. D., Devillers-Thiery A., Bernardi G. Specificity of spleen acid DNAase. Eur J Biochem. 1973 Oct 18;38(3):434–442. doi: 10.1111/j.1432-1033.1973.tb03077.x. [DOI] [PubMed] [Google Scholar]
  15. WEISSMANN C., BILLETER M. A., SCHNEIDER M. C., KNIGHT C. A., OCHOA S. REPLICATION OF VIRAL RNA. VI. NUCLEOTIDE COMPOSITION OF THE REPLICATIVE FORM OF TOBACCO MOSAIC VIRUS RNA AND OF ITS COMPONENT STRANDS. Proc Natl Acad Sci U S A. 1965 Mar;53:653–656. doi: 10.1073/pnas.53.3.653. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES