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. 1974 Feb;1(2):267–278. doi: 10.1093/nar/1.2.267

Physical consequences of chemical modification: circular dichroism of the kethoxalated oligoribonucleotides

AI Poletayev 1, TA Avdonina 1, LL Kisselev 1
PMCID: PMC343345  PMID: 4415066

Abstract

Alterations in CD spectra are found in G-containing oligoribonucleotides after modification with kethoxal (β-ethoxy--α-ketobutyraldehyde). Stacking interactions in kethoxalated oligomers are followed by temperature dependence of their CD amplitudes. It is shown that for oligomers with nucleosides in anti-conformation adduct formation destroys the stacking interaction with 3′-neighbour but not with a 5′-neighbour. For nucleosides in non-standard conformation (i.e. syn-conformation of guanine in GpGpCp) the physical alteractions may be seen in those cases, when the substituting group affects the initial conformation or the interplane base contacts via, for instance, blocking NH2-group of guanine in GpUp.

The results demonstrated that even a single monomer modification in a polymer chain could not be considered as a local event having no influence on the three-dimensional structure. The degree of conformational disorders depends both on the conformation of single nucleotides in the stack and on the nature of the nearest neighbours of the modified base.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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