Skip to main content

Some NLM-NCBI services and products are experiencing heavy traffic, which may affect performance and availability. We apologize for the inconvenience and appreciate your patience. For assistance, please contact our Help Desk at info@ncbi.nlm.nih.gov.

Nucleic Acids Research logoLink to Nucleic Acids Research
. 1974 May;1(5):707–718. doi: 10.1093/nar/1.5.707

Transfer RNA methylating activity of yeast mitochondria

Nina Smolar 1, Ingvar Svensson 1
PMCID: PMC343371  PMID: 10793751

Abstract

Mitochondria isolated from Saccharomyces cerevisiae and purified in Urografin or sucrose gradient contain tRNA methylating activity with specificities different from those of the cytoplasm. The main reaction product, using E.coli tRNA as methyl group acceptor, is N2,-N2-dimethylguanine. The corresponding mitochondrial methylase is coded by nuclear DNA. A DNA methylating activity is also associated with yeast mitochondria.

Full text

PDF
707

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Accoceberry B., Schneller J. M., Stahl A. J. Etude comparative des valyl tRNA cytoplasmique et mitochondrial de levure. Biochimie. 1973;55(3):291–296. doi: 10.1016/s0300-9084(73)80128-2. [DOI] [PubMed] [Google Scholar]
  2. Accoceberry B., Stahl A. Chromatographic differences between the cytoplasmic and the mitochondrial t-RNA of Saccharomyces cerevisiae. Biochem Biophys Res Commun. 1971 Mar 19;42(6):1235–1243. doi: 10.1016/0006-291x(71)90038-6. [DOI] [PubMed] [Google Scholar]
  3. Ashwell M., Work T. S. The biogenesis of mitochondria. Annu Rev Biochem. 1970;39:251–290. doi: 10.1146/annurev.bi.39.070170.001343. [DOI] [PubMed] [Google Scholar]
  4. Barnett W. E., Brown D. H., Epler J. L. Mitochondrial-specific aminoacyl-RNA synthetases. Proc Natl Acad Sci U S A. 1967 Jun;57(6):1775–1781. doi: 10.1073/pnas.57.6.1775. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Björk G. R., Neidhardt F. C. Evidence for the utilization of host tRNA(m 5 U)methylase to modify tRNA coded by phage T4. Virology. 1973 Apr;52(2):507–519. doi: 10.1016/0042-6822(73)90346-2. [DOI] [PubMed] [Google Scholar]
  6. Björk G. R., Svensson I. Analysis of methylated constituents from RNA by thin-layer chromatography. Biochim Biophys Acta. 1967 Apr 18;138(2):430–432. doi: 10.1016/0005-2787(67)90504-7. [DOI] [PubMed] [Google Scholar]
  7. Borst P. Mitochondrial nucleic acids. Annu Rev Biochem. 1972;41:333–376. doi: 10.1146/annurev.bi.41.070172.002001. [DOI] [PubMed] [Google Scholar]
  8. Casey J., Cohen M., Rabinowitz M., Fukuhara H., Getz G. S. Hybridization of mitochondrial transfer RNA's with mitochondrial and nuclear DNA of grande (wild type) yeast. J Mol Biol. 1972 Feb 14;63(3):431–440. doi: 10.1016/0022-2836(72)90438-x. [DOI] [PubMed] [Google Scholar]
  9. Dubin D. T., Friend D. A. Degree of methylation of mitochondrial ribosomal RNA. FEBS Lett. 1971 Nov 1;18(2):287–289. doi: 10.1016/0014-5793(71)80467-2. [DOI] [PubMed] [Google Scholar]
  10. Dubin D. T., Montenecourt B. S. Mitochondrial RNA from cultured animal cells. Distinctive high-molecular-weight and 4 s species. J Mol Biol. 1970 Mar 14;48(2):279–295. doi: 10.1016/0022-2836(70)90161-0. [DOI] [PubMed] [Google Scholar]
  11. Guarnieri M., Mattoon J. R., Balcavage W. X., Payne C. A rapid semimicro method for production of yeast mitochondria. Anal Biochem. 1970 Mar;34(1):39–45. doi: 10.1016/0003-2697(70)90084-9. [DOI] [PubMed] [Google Scholar]
  12. Guderian R. H., Pulliam R. L., Gordon M. P. Characterization and fractionation of tobacco leaf transfer RNA. Biochim Biophys Acta. 1972 Feb 23;262(1):50–65. doi: 10.1016/0005-2787(72)90218-3. [DOI] [PubMed] [Google Scholar]
  13. Halbreich A., Rabinowitz M. Isolation of Saccharomyces cerevisiae mitochondrial formyltetrahydrofolic acid:methionyl-tRNA transformylase and the hybridization of mitochondrial fMet-tRNA with mitochondrial DNA. Proc Natl Acad Sci U S A. 1971 Feb;68(2):294–298. doi: 10.1073/pnas.68.2.294. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Isaksson L. A., Phillips J. H. Studies on microbial RNA. V. A comparison of the in vivo methylated components of ribosomal RNA from Escherichia coli and Saccharomyces cerevisiae. Biochim Biophys Acta. 1968 Jan 29;155(1):63–71. [PubMed] [Google Scholar]
  15. Klagsbrun M. Differences in the methylation of transfer ribonucleic acid in vitro by the mitochondrial and cytoplasmic transfer ribonucleic acid methylases of HeLa cells. J Biol Chem. 1973 Apr 10;248(7):2606–2611. [PubMed] [Google Scholar]
  16. Leon S. A., Mahler H. R. Isolation and properties of mitochondrial RNA from yeast. Arch Biochem Biophys. 1968 Jul;126(1):305–319. doi: 10.1016/0003-9861(68)90587-0. [DOI] [PubMed] [Google Scholar]
  17. MANDEL L. R., BOREK E. THE NATURE OF THE RNA SYNTHESIZED DURING CONDITIONS OF UNBALANCED GROWTH IN E. COLI K12W-6. Biochemistry. 1963 May-Jun;2:560–566. doi: 10.1021/bi00903a030. [DOI] [PubMed] [Google Scholar]
  18. Morimoto H., Halvorson H. O. Characterization of mitochondrial ribosomes from yeast. Proc Natl Acad Sci U S A. 1971 Feb;68(2):324–328. doi: 10.1073/pnas.68.2.324. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Mukerji S. K., Deutscher M. P. Reactions at the 3' terminus of transfer ribonucleic acid. V. Subcellular localization and evidence for a mitochondrial transfer ribonucleic acid nucleotidyltransferase. J Biol Chem. 1972 Jan 25;247(2):481–488. [PubMed] [Google Scholar]
  20. Nass M. M. Differential methylation of mitochondrial and nuclear DNA in cultured mouse, hamster and virus-transformed hamster cells. In vivo and in vitro methylation. J Mol Biol. 1973 Oct 15;80(1):155–175. doi: 10.1016/0022-2836(73)90239-8. [DOI] [PubMed] [Google Scholar]
  21. Rabinowitz M., Swift H. Mitochondrial nucleic acids and their relation to the biogenesis of mitochondria. Physiol Rev. 1970 Jul;50(3):376–427. doi: 10.1152/physrev.1970.50.3.376. [DOI] [PubMed] [Google Scholar]
  22. Richter D., Lipmann F. Separation of mitochondrial and cytoplasmic peptide chain elongation factors from yeast. Biochemistry. 1970 Dec 22;9(26):5065–5070. doi: 10.1021/bi00828a004. [DOI] [PubMed] [Google Scholar]
  23. SVENSSON I., BOMAN H. G., ERIKSSON K. G., KJELLIN K. STUDIES ON MICROBIAL RNA. I. TRANSFER OF METHYL GROUPS FROM METHIONINE TO SOLUBLE RNA FROM ESCHERICHIA COLI. J Mol Biol. 1963 Sep;7:254–271. doi: 10.1016/s0022-2836(63)80006-6. [DOI] [PubMed] [Google Scholar]
  24. Scragg Alan H. Chain elongation factors of yeast mitochondria. FEBS Lett. 1971 Sep 15;17(1):111–114. doi: 10.1016/0014-5793(71)80576-8. [DOI] [PubMed] [Google Scholar]
  25. Svensson I., Björk G. R., Lundahl P. Studies on microbial RNA. Properties of tRNA methylases from Saccharomyces cerevisiae. Eur J Biochem. 1969 Jun;9(2):216–221. doi: 10.1111/j.1432-1033.1969.tb00597.x. [DOI] [PubMed] [Google Scholar]
  26. Svensson I., Isaksson L., Henningsson A. Aminoacylation and polypeptide synthesis with tRNA lacking ribothymidine. Biochim Biophys Acta. 1971 May 13;238(2):331–337. doi: 10.1016/0005-2787(71)90100-6. [DOI] [PubMed] [Google Scholar]
  27. Svensson I. Studies on methionyl-tRNA synthetase. I. Effects of divalent and monovalent cations on methionyl-tRNA synthetase from Saccharomyces cerevisiae. Biochim Biophys Acta. 1967 Sep 12;146(1):239–252. doi: 10.1016/0005-2744(67)90090-3. [DOI] [PubMed] [Google Scholar]
  28. Tsai M. J., Michaelis G., Criddle R. S. DNA-dependent RNA polymerase from yeast mitochondria. Proc Natl Acad Sci U S A. 1971 Feb;68(2):473–477. doi: 10.1073/pnas.68.2.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Wintersberger U., Wintersberger E. Studies on deoxyribonucleic acid polymerases from yeast. 2. Partial purification and characterization of mitochondrial DNA polymerase from wild type and respiration-deficient yeast cells. Eur J Biochem. 1970 Mar 1;13(1):20–27. doi: 10.1111/j.1432-1033.1970.tb00894.x. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES