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. 1974 Jun;1(6):727–752. doi: 10.1093/nar/1.6.727

The mapping and ordering of fragments of SV40 DNA produced by restriction endonucleases

KN Subramanian 1, J Pan 1, S Zain 1, SM Weissman 1
PMCID: PMC343376  PMID: 10793753

Abstract

SV40 DNA is cleaved by the Eco RII and Hae restriction endonucleases to give rise to two different sets of 16 fragments each. These fragments have been ordered by analysis of the products of redigestion of one set of fragments with another restriction enzyme. The cleavage sites have been precisely mapped based on length measurements of the products of each cleavage. This provides a convenient group of small DNA fragments suitable for sequence analysis investigation of the transcripts present in infected cells, or construction of deletion substitution variants of the virus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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