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. 1974 Jun;1(6):787–801. doi: 10.1093/nar/1.6.787

Hybrid isolation by recovery of RNA-DNA hybrids from agar using S1 nuclease

JNorman Hansen 1, Barbara H Pheiffer 1, Christopher J Hough 1
PMCID: PMC343380  PMID: 10793757

Abstract

A method for recovering RNA-DNA hybrids from agar employing a single strand specific nuclease is described. The procedure is suitable for large scale isolations, and immobilization of the DNA in agar prior to hybridization allows a high yield of hybrid without interference by DNA reannealing.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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