Figure 5.

5-Thiomannosides significantly inhibit the biosynthesis of dolichol-linked oligosaccharides. (A) Solvent partitioning of cell extracts into organic or aqueous solvents demonstrate that reduced amounts of [³H]-mannose are incorporated into cells treated with the 1 – 3. The [³H] activity of the DLO pools are reported as a fraction of the total amount of intracellular activity. N = 3 for each condition and the data are reported ± standard deviation. p–values were calculated using the unpaired, two-tailed student’s t-test. Fluorophore-assisted carbohydrate electrophoresis (FACE) analysis of DLOs extracted from (B) CHO K1 and (C) SRD-12B cells demonstrates that in both cell lines 1 and 2 reduced DLO biosynthesis relative to non-treated cells. The positions of standards i – iii are established through the use of purified standards. G4 – G7 refer to dextran oligomers of four to seven glucose units.