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. 1974 Jul;1(7):889–899. doi: 10.1093/nar/1.7.889

The effect of bisulfite modification on the template activity of DNA for DNA polymerase I1

Kazushige Kai 1,a, Takashi Tsuruo 1, Hikoya Hayatsu 1
PMCID: PMC343392  PMID: 10793721

Abstract

Cytosine residues of poly(C) and heat-denatured calf thymus DNA were transformed into 5,6-dihydrouracil-6-sulfonate (U(SO−3)) residues by treatment with bisulfite. The poly(U(SO−3)2, C3) and poly(U(SO−3)9, C1) prepared did not form inter-base binding with either poly(A) or poly(I) as judged by the absence of hypochromicity in ultraviolet absorbance. U(SO−3) residues in the DNA inactivated it to serve as template for E.coli DNA polymerase I, while the template activity was restored by conversion of the U(SO−3) residues into U.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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