Skip to main content
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1974 Nov;1(11):1411–1419. doi: 10.1093/nar/1.11.1411

Repeated DNA sequences in fungi

SK Dutta 1
PMCID: PMC343421  PMID: 10793700

Abstract

Several fungal species, representatives of all broad groups like basidiomycetes, ascomycetes and phycomycetes, were examined for the nature of repeated DNA sequences by DNA:DNA reassociation studies using hydroxyapatite chromatography. All of the fungal species tested contained 10-20% repeated DNA sequences. There are approximately 100-110 copies of repeated DNA sequences of approximately 4 × 107 daltons piece size of each. Repeated DNA sequence homoduplexes showed on average 5°C difference of Te50 (temperature at which 50% duplexes dissociate) values from the corresponding homoduplexes of unfractionated whole DNA. It is suggested that a part of repetitive sequences in fungi constitutes mitochondrial DNA and a part of it constitutes nuclear DNA.

Full text

PDF
1411

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Britten R. J., Davidson E. H. Gene regulation for higher cells: a theory. Science. 1969 Jul 25;165(3891):349–357. doi: 10.1126/science.165.3891.349. [DOI] [PubMed] [Google Scholar]
  2. Britten R. J., Graham D. E., Neufeld B. R. Analysis of repeating DNA sequences by reassociation. Methods Enzymol. 1974;29:363–418. doi: 10.1016/0076-6879(74)29033-5. [DOI] [PubMed] [Google Scholar]
  3. Britten R. J., Kohne D. E. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms. Science. 1968 Aug 9;161(3841):529–540. doi: 10.1126/science.161.3841.529. [DOI] [PubMed] [Google Scholar]
  4. Brooks R. R., Huang P. C. Redundant DNA of Neurospora crassa. Biochem Genet. 1972 Feb;6(1):41–49. doi: 10.1007/BF00485964. [DOI] [PubMed] [Google Scholar]
  5. Brown D. D., Wensink P. C., Jordan E. A comparison of the ribosomal DNA's of Xenopus laevis and Xenopus mulleri: the evolution of tandem genes. J Mol Biol. 1972 Jan 14;63(1):57–73. doi: 10.1016/0022-2836(72)90521-9. [DOI] [PubMed] [Google Scholar]
  6. Chattopadhyay S. K., Kohne D. E., Dutta S. K. Ribosomal RNA genes of Neurospora: isolation and characterization. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3256–3259. doi: 10.1073/pnas.69.11.3256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Crippa M., Meza I., Dina D. Sequence arrangement in mRNA: presence of poly(A) and identification of a repetitive fragment at the 5' end. Cold Spring Harb Symp Quant Biol. 1974;38:933–942. doi: 10.1101/sqb.1974.038.01.095. [DOI] [PubMed] [Google Scholar]
  8. Dutta S. K., Ojha M. Relatedness between major taxonomic groups of fungi based on the measurement of DNA nucleotide sequence homology. Mol Gen Genet. 1972;114(3):232–240. doi: 10.1007/BF01788892. [DOI] [PubMed] [Google Scholar]
  9. Dutta S. K., Penn S. R., Knight A. R., Ojha M. Characterization of DNAs from coprinus lagopus and mucor azygospora. Experientia. 1972 May 15;28(5):582–584. doi: 10.1007/BF01931892. [DOI] [PubMed] [Google Scholar]
  10. Dutta S. K., Richman N., Woodward V. W., Mandel M. Relatedness among species of fungi and higher plants measured by DNA hybridization and base ratios. Genetics. 1967 Nov;57(3):719–727. doi: 10.1093/genetics/57.3.719. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Finkelstein D. B., Blamire J., Marmur J. Isolation and fractionation of yeast nucleic acids. II. Rapid isolation of mitochondrial deoxyribonucleic acid by poly(L-lysine) kieselguhr chromatography. Biochemistry. 1972 Dec 5;11(25):4853–4858. doi: 10.1021/bi00775a033. [DOI] [PubMed] [Google Scholar]
  12. Hough B. R., Davidson E. H. Studies on the repetitive sequence transcripts of Xenopus oocytes. J Mol Biol. 1972 Oct 14;70(3):491–509. doi: 10.1016/0022-2836(72)90555-4. [DOI] [PubMed] [Google Scholar]
  13. Ojha M., Turian G. Interspecific transformation and DNA characteristics in Allomyces. Mol Gen Genet. 1971;112(1):49–59. doi: 10.1007/BF00266932. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES