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. 1974 Nov;1(11):1587–1599. doi: 10.1093/nar/1.11.1587

Some general methods of preparing affinity columns

PV Sundaram 1
PMCID: PMC343438  PMID: 10793714

Abstract

Some general methods of covalent coupling of nucleotides, especially derivatized nucleotides, polynucleotides and cofactors to insoluble polymers are described in this paper. Wherever necessary individual methods also carry some information on the binding of enzymes to the same polymers to serve as a guide to the efficiency of the coupling methods.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson G. W., Zimmerman J. E., Callahan F. M. A reinvestigation of the mixed carbonic anhydride method of peptide synthesis. J Am Chem Soc. 1967 Sep 13;89(19):5012–5017. doi: 10.1021/ja00995a032. [DOI] [PubMed] [Google Scholar]
  2. Axén R., Porath J., Ernback S. Chemical coupling of peptides and proteins to polysaccharides by means of cyanogen halides. Nature. 1967 Jun 24;214(5095):1302–1304. doi: 10.1038/2141302a0. [DOI] [PubMed] [Google Scholar]
  3. Berglund O., Eckstein F. Synthesis of ATP- and dATP-substituted sepharoses and their application in the purification of phage-T4-induced ribonucleotide reductase. Eur J Biochem. 1972 Aug 4;28(4):492–496. doi: 10.1111/j.1432-1033.1972.tb01936.x. [DOI] [PubMed] [Google Scholar]
  4. Dean P. D., Lowe C. R. Some studies on insolubilized nicotinamide nucleotides. Biochem J. 1972 Apr;127(2):11P–12P. doi: 10.1042/bj1270011p. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Eckstein F., Gindl H. Polyribonucleotides containing a phosphorothioate backbone. Eur J Biochem. 1970 Apr;13(3):558–564. doi: 10.1111/j.1432-1033.1970.tb00961.x. [DOI] [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Lowe C. R., Harvey M. J., Craven D. B., Dean P. D. Some parameters relevant to affinity chromatography on immobilized nucleotides. Biochem J. 1973 Jul;133(3):499–506. doi: 10.1042/bj1330499. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. MICHELSON A. M. SYNTHESIS OF NUCLEOTIDE ANHYDRIDES BY ANION EXCHANGE. Biochim Biophys Acta. 1964 Sep 11;91:1–13. doi: 10.1016/0926-6550(64)90164-1. [DOI] [PubMed] [Google Scholar]
  9. Mosbach K., Guilford H., Ohlsson R., Scott M. General ligands in affinity chromatography. Cofactor-substrate elution of enzymes bound to the immobilized nucleotides adenosine 5'-monophosphate and nicotinamide-adenine dinucleotide. Biochem J. 1972 May;127(4):625–631. doi: 10.1042/bj1270625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Poonian M. S., Schlabach A. J., Weissbach A. Covalent attachment of nucleic acids to agarose for affinity chromatography. Biochemistry. 1971 Feb 2;10(3):424–427. doi: 10.1021/bi00779a011. [DOI] [PubMed] [Google Scholar]
  11. Sundaram P. V., Hornby W. E. Preparation and properties of urease chemically attached to nylon tube. FEBS Lett. 1970 Oct;10(5):325–327. doi: 10.1016/0014-5793(70)80464-1. [DOI] [PubMed] [Google Scholar]
  12. Wagner A. F., Bugianesi R. L., Shen T. Y. Preparation of sepharose-bound poly (rI:rC). Biochem Biophys Res Commun. 1971 Oct 1;45(1):184–189. doi: 10.1016/0006-291x(71)90067-2. [DOI] [PubMed] [Google Scholar]
  13. Wider de Xifra E. A., Mendiara S., Batlle A. M. Studies on the reaction mechanism of soybean callus succinyl CoA synthetase. Phosphorylation of the enzyme on immobilized adenosine-triphosphate and enzyme attached to a solid support. FEBS Lett. 1972 Nov 1;27(2):275–278. doi: 10.1016/0014-5793(72)80639-2. [DOI] [PubMed] [Google Scholar]

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