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. 1974 Dec;1(12):1631–1647. doi: 10.1093/nar/1.12.1631

Enzymatic synthesis of deoxyribo-oligonucleotides of defined sequence. Properties of the enzyme*

Shirley Gillam 1, Michael Smith 1
PMCID: PMC343444  PMID: 4615301

Abstract

A modified purification is described for an enzyme, from Escherichia coli B, which polymerizes deoxyribonucleoside-5′ diphosphates. Under appropriate conditions, the enzyme will add a single deoxyribonucleotide residue to a deoxyribo-oligonucleotide primer. At all stages, the enzyme activity copurified with the activity which will polymerize adenosine-5′ diphosphate (polynucleotide phosphorylase). Studies of heat stability, the effect of various temperatures of reaction and of disc gel electrophoresis failed to provide evidence that the two activities are separable.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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