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. 1974 Dec;1(12):1713–1720.

The phenylalanine tRNA from Mycoplasma sp. (Kid): a tRNA lacking hypermodified nucleosides functional in protein synthesis

Margaret E Kimball 1, Dieter Söll 1
PMCID: PMC343450  PMID: 4615304

Abstract

Phenylalanine tRNA from Mycoplasma sp. (Kid) was purified and characterized. The tRNA can be aminoacylated by phenylalanyl-tRNA synthetase from both Mycoplasma and E. coli. In a tRNA-dependent cell-free E. coli amino acid incorporating system programmed with poly U pure Mycoplasma tRNAPhe was fully active in promoting phenylalanine incorporation, even in direct competition with homologous E. coli tRNAPhe. Since the Mycoplasma tRNA lacks isopentenyladenosine, or any related hypermodified nucleoside, it appears that the presence of such nucleosides in tRNA is not an absolute requirement for protein synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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