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. 1974 Dec;1(12):1733–1738. doi: 10.1093/nar/1.12.1733

Sequence studies of nonradioactive Mycoplasma tRNAPhe with the aid of polynucleotide phosphorylase and polynucleotide kinase

Kwok S Szeto 1, Dieter Söll 1
PMCID: PMC343452  PMID: 4449733

Abstract

The known methods of enzymatic phosphorylation with [32P]phosphate of the 3′- or 5′-hydroxyl group of an oligonucleotide have been applied to oligonucleotides derived from Mycoplasma tRNAPhe. The fingerprints obtained by both methods are very similar to each other and to that of uniformly labelled tRNA. The sequence of some oligonucleotides was determined by partial digestion of the 3′-phosphorylated fragment with spleen phosphodiesterase and of the corresponding 5′-phosphorylated fragment with venom phosphodiesterase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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