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. 1975 Jun;2(6):821–830. doi: 10.1093/nar/2.6.821

Ribosome-dependent conversion of polyA-containing heterogenous nuclear RNA into smaller RNA molecules.

J Grozdanovic, J Hradec
PMCID: PMC343469  PMID: 1144064

Abstract

The polyA-containing heterogenous nuclear RNA fraction separated from total rat liver nRNA by gel filtration on Sepharose 4B followed by affinity chromatography on polyU-Sepharose and containing predominantly the 45S components becomes enzymatically bound to homologous 80S ribosomes and polyribosomes at 0 degree C. If 80S ribosomes or polyribosomes with bound poly-a-containing HnRNA are subjected to a further incubation at 37 degree C, the original 45S RNA is gradually converted into smaller RNA species of 10- 35S which remain bound to the particle. This ribosome-dependent cleavage of larger HnRNA species into smaller RNA molecules may represent the ultimate step of mRNA maturation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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