Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1975 Jun;2(6):839–852. doi: 10.1093/nar/2.6.839

Determination of the sequences of 18 nucleotides from the 5'-end of the 1-strand and 15 nucleotides from the 5'-end of the r-strand of T7 DNA.

P C Loewen
PMCID: PMC343471  PMID: 167354

Abstract

The sequences of 18 nucleotides from the 5'-end of the 1-strand and 15 nucleotides from the 5'-end of the r-strand of T7 bacteriophage DNA have been determined to be pT-C-T-C-A-C-A-G-T-G-T-A-C-G-T-C-C-C (1-strand) and pA-G-G-G-A-C-A-C-A-G-C-G-C-T-C (r-strand). The 5'-termini of whole DNA or separated strands were kinased using polynucleotide kinase and (gamma-32-P) rATP. The DNA was partially digested with pancreatic DNase and the fragments were separated by two dimensional electrophoresis and homochromatography. To complete the sequence, snake venom phosphodiesterase digestions of these fragments were carried out. The relationship of these sequences to the proposed cleavage of concatemeric DNA during DNA replication is discussed.

Full text

PDF
839

Images in this article

843Image
on p.843
844Image
on p.844
845Image
on p.845
846Image
on p.846

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bambara R., Jay E., Wu R. DNA sequence analysis: a formula to predict electrophoretic mobilities of oligonucleotides on cellulose acetate. Nucleic Acids Res. 1974 Nov;1(11):1503–1520. doi: 10.1093/nar/1.11.1503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Englund P. T. The 3'-terminal nucleotide sequences of T7 DNA. J Mol Biol. 1972 May 14;66(2):209–224. doi: 10.1016/0022-2836(72)90474-3. [DOI] [PubMed] [Google Scholar]
  3. Gilbert W., Maxam A. The nucleotide sequence of the lac operator. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3581–3584. doi: 10.1073/pnas.70.12.3581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Glynn I. M., Chappell J. B. A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity. Biochem J. 1964 Jan;90(1):147–149. doi: 10.1042/bj0900147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jay E., Bambara R., Padmanabhan R., Wu R. DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping. Nucleic Acids Res. 1974 Mar;1(3):331–353. doi: 10.1093/nar/1.3.331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Loewen P. C., Sekiya T., Khorana H. G. The nucleotide sequence adjoining the CCA end of an Escherichia coli tyrosine transfer ribonucleic acid gene. J Biol Chem. 1974 Jan 10;249(1):217–226. [PubMed] [Google Scholar]
  7. Maniatis T., Ptashne M., Barrell B. G., Donelson J. Sequence of a repressor-binding site in the DNA of bacteriophage lamda. Nature. 1974 Aug 2;250(465):394–397. doi: 10.1038/250394a0. [DOI] [PubMed] [Google Scholar]
  8. Panet A., van de Sande J. H., Loewen P. C., Khorana H. G., Raae A. J., Lillehaug J. R., Kleppe K. Physical characterization and simultaneous purification of bacteriophage T4 induced polynucleotide kinase, polynucleotide ligase, and deoxyribonucleic acid polymerase. Biochemistry. 1973 Dec 4;12(25):5045–5050. doi: 10.1021/bi00749a003. [DOI] [PubMed] [Google Scholar]
  9. Price S. S., Schwing J. M., Englund P. T. The 3'-terminal heptanucleotide sequence of the l strand of T7 deoxyribonucleic acid. J Biol Chem. 1973 Oct 25;248(20):7001–7006. [PubMed] [Google Scholar]
  10. Ritchie D. A., Thomas C. A., Jr, MacHattie L. A., Wensink P. C. Terminal repetition in non-permuted T3 and T7 bacteriophage DNA molecules. J Mol Biol. 1967 Feb 14;23(3):365–376. doi: 10.1016/s0022-2836(67)80111-6. [DOI] [PubMed] [Google Scholar]
  11. Sanger F., Donelson J. E., Coulson A. R., Kössel H., Fischer D. Use of DNA polymerase I primed by a synthetic oligonucleotide to determine a nucleotide sequence in phage fl DNA. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1209–1213. doi: 10.1073/pnas.70.4.1209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sekiua T., Ormondt H. V., Khorana H. G. The nucleotide sequence in the promoter region of the fene for an Escherichia coli tyrosine transfer ribonucleic acid. J Biol Chem. 1975 Feb 10;250(3):1087–1098. [PubMed] [Google Scholar]
  13. Sekiya T., Khorana H. G. Nucleotide sequence in the promoter region of the Escherichia coli tyrosine tRNA gene. Proc Natl Acad Sci U S A. 1974 Aug;71(8):2978–2982. doi: 10.1073/pnas.71.8.2978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sgaramella V., Van de Sande J. H., Khorana H. G. Studies on polynucleotides, C. A novel joining reaction catalyzed by the T4-polynucleotide ligase. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1468–1475. doi: 10.1073/pnas.67.3.1468. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Watson J. D. Origin of concatemeric T7 DNA. Nat New Biol. 1972 Oct 18;239(94):197–201. doi: 10.1038/newbio239197a0. [DOI] [PubMed] [Google Scholar]
  16. Weiss B., Live T. R., Richardson C. C. Enzymatic breakage and joining of deoxyribonucleic acid. V. End group labeling and analysis of deoxyribonucleic acid containing single straned breaks. J Biol Chem. 1968 Sep 10;243(17):4530–4542. [PubMed] [Google Scholar]
  17. Weiss B., Richardson C. C. The 5'-terminal dinucleotides of the separated strands of T7 bacteriophage deoxyribonucleic acid. J Mol Biol. 1967 Feb 14;23(3):405–415. doi: 10.1016/s0022-2836(67)80115-3. [DOI] [PubMed] [Google Scholar]
  18. Wu R., Taylor E. Nucleotide sequence analysis of DNA. II. Complete nucleotide sequence of the cohesive ends of bacteriophage lambda DNA. J Mol Biol. 1971 May 14;57(3):491–511. doi: 10.1016/0022-2836(71)90105-7. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES