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. 1975 Jun;2(6):905–913. doi: 10.1093/nar/2.6.905

Isolation of endonuclease from mammalian cells by DNA - cellulose chromatography.

A Caputo, G Zupi, A Clanciulli
PMCID: PMC343477  PMID: 1144065

Abstract

By means of DNA-cellulose chromatography an enzyme with endonucleolytic activity has been isolated from nuclear acidic protein fraction of mammalian cells. The main active fraction, eluted at 0.7 M NaCl, effects the velocity sedimentation of UV-irradiated and alkylated DNA, resulting in a decrease of the molecular weight. The fraction is completely inactive using native as well as heat-denatured DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blobel G., Potter V. R. Nuclei from rat liver: isolation method that combines purity with high yield. Science. 1966 Dec 30;154(3757):1662–1665. doi: 10.1126/science.154.3757.1662. [DOI] [PubMed] [Google Scholar]
  2. Friedberg E. C., Goldthwait D. A. Endonuclease II of E. coli. Cold Spring Harb Symp Quant Biol. 1968;33:271–275. doi: 10.1101/sqb.1968.033.01.031. [DOI] [PubMed] [Google Scholar]
  3. Grossman L., Kaplan J. C., Kushner S. R., Mahler I. Enzymes involved in the early stages of repair of ultraviolet-irradiated DNA. Cold Spring Harb Symp Quant Biol. 1968;33:229–234. doi: 10.1101/sqb.1968.033.01.026. [DOI] [PubMed] [Google Scholar]
  4. Kaplan J. C., Kushner S. R., Grossman L. Enzymatic repair of DNA, 1. Purification of two enzymes involved in the excision of thymine dimers from ultraviolet-irradiated DNA. Proc Natl Acad Sci U S A. 1969 May;63(1):144–151. doi: 10.1073/pnas.63.1.144. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lindahl T. Excision of pyrimidine dimers from ultraviolet-irradiated DNA by exonucleases from mammalian cells. Eur J Biochem. 1971 Feb 1;18(3):407–414. doi: 10.1111/j.1432-1033.1971.tb01257.x. [DOI] [PubMed] [Google Scholar]
  6. Litman R. M. A deoxyribonucleic acid polymerase from Micrococcus luteus (Micrococcus lysodeikticus) isolated on deoxyribonucleic acid-cellulose. J Biol Chem. 1968 Dec 10;243(23):6222–6233. [PubMed] [Google Scholar]
  7. Moriguchi E., Suzuki K. Enzymatic breakdown of UV-irradiated DNA by the extract from Micrococcus lysodeicticus. Biochem Biophys Res Commun. 1966 Jul 20;24(2):195–202. doi: 10.1016/0006-291x(66)90719-4. [DOI] [PubMed] [Google Scholar]
  8. Okubo S., Nakayama H., Takagi Y. Repair of ultraviolet-damaged DNA in Micrococcus lysodeikticus. II. In vivo investigation on endonuclease activity specific for ultraviolet-irradiated DNA. Biochim Biophys Acta. 1971 Jan 1;228(1):83–94. [PubMed] [Google Scholar]
  9. Ramstein J., Héléne C., Leng M. A study of chemically methylated deoxyribonucleic acid. Eur J Biochem. 1971 Jul 15;21(1):125–136. doi: 10.1111/j.1432-1033.1971.tb01448.x. [DOI] [PubMed] [Google Scholar]
  10. STRAUSS B. S. Differential destruction of the transforming activity of damaged deoxyribonucleic acid by a bacterial enzyme. Proc Natl Acad Sci U S A. 1962 Sep 15;48:1670–1675. doi: 10.1073/pnas.48.9.1670. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. STUDIER F. W. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA. J Mol Biol. 1965 Feb;11:373–390. doi: 10.1016/s0022-2836(65)80064-x. [DOI] [PubMed] [Google Scholar]
  12. Strauss B., Coyle M., Robbins M. Alkylation damage and its repair. Cold Spring Harb Symp Quant Biol. 1968;33:277–287. doi: 10.1101/sqb.1968.033.01.032. [DOI] [PubMed] [Google Scholar]

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