Abstract
After mild trypsin treatment of a complex of ribosomal protein S4 and 16S RNA of Escherichia coli, a large homogeneous fragment of the S4 protein was protected against digestion by its RNA binding site. This fragment was isolated and characterised for molecular weight. It was able to rebind specifically to 16S RNA. Preliminary results indicate that protected protein fragments can also be obtained from other proteins that complex specifically with 23S and 5S RNA.
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- Amons R., Möller W., Schiltz E., Reinbolt J. Studies on the binding sites of protein S4 to 16S RNA in Escherichia coli ribosomes. FEBS Lett. 1974 Apr 15;41(1):135–138. doi: 10.1016/0014-5793(74)80972-5. [DOI] [PubMed] [Google Scholar]
- Daya-Grosjean L., Garrett R. A., Pongs O., Stöffler G., Wittmann H. G. Properties of the interaction of ribosomal protein S4 and 16S RNA in Escherichia coli revertants from Streptomycin dependence to independence. Mol Gen Genet. 1972;119(3):277–286. doi: 10.1007/BF00333865. [DOI] [PubMed] [Google Scholar]
- Daya-Grosjean L., Reinbolt J., Pongs O., Garrett R. A. A study of the regions of ribosomal proteins S4, S8, S15 and S20 that interact with 16 S RNA of Escherichia coli. FEBS Lett. 1974 Aug 30;44(3):253–256. doi: 10.1016/0014-5793(74)81151-8. [DOI] [PubMed] [Google Scholar]
- Ehresmann C., Stiegler P., Mackie G. A., Zimmermann R. A., Ebel J. P., Fellner P. Primary sequence of the 16S ribosomal RNA of Escherichia coli. Nucleic Acids Res. 1975 Feb;2(2):265–278. doi: 10.1093/nar/2.2.265. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Funatsu G., Puls W., Schiltz E., Reinbolt J., Wittmann H. G. Ribosomal proteins. XXXI. Comparative studies on altered proteins S4 of six Escherichia coli revertants from streptomycin dependence. Mol Gen Genet. 1972;115(2):131–139. doi: 10.1007/BF00277293. [DOI] [PubMed] [Google Scholar]
- Garrett R. A., Rak K. H., Daya L., Stöffler G. Ribosomal proteins. XXIX. Specific protein binding sites on 16S rRNA of Escherichia coli. Mol Gen Genet. 1972;114(2):112–124. doi: 10.1007/BF00332782. [DOI] [PubMed] [Google Scholar]
- Hardy S. J., Kurland C. G., Voynow P., Mora G. The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins. Biochemistry. 1969 Jul;8(7):2897–2905. doi: 10.1021/bi00835a031. [DOI] [PubMed] [Google Scholar]
- Hindennach I., Stöffler G., Wittmann H. G. Ribosomal proteins. Isolation of the proteins from 30S ribosomal subunits of Escherichia coli. Eur J Biochem. 1971 Nov 11;23(1):7–11. doi: 10.1111/j.1432-1033.1971.tb01584.x. [DOI] [PubMed] [Google Scholar]
- Kaltschmidt E., Wittmann H. G. Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins. Anal Biochem. 1970 Aug;36(2):401–412. doi: 10.1016/0003-2697(70)90376-3. [DOI] [PubMed] [Google Scholar]
- Lake J. A., Pendergast M., Kahan L., Nomura M. Localization of Escherichia coli ribosomal proteins S4 and S14 by electron microscopy of antibody-labeled subunits. Proc Natl Acad Sci U S A. 1974 Dec;71(12):4688–4692. doi: 10.1073/pnas.71.12.4688. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lemieux G. Chemical modifications of Escherichia coli ribosomal proteins S4, S7, and S8 in regard to their capacity for binding 16 S RNA. Can J Biochem. 1974 Nov;52(11):1038–1043. doi: 10.1139/o74-145. [DOI] [PubMed] [Google Scholar]
- Mizushima S., Nomura M. Assembly mapping of 30S ribosomal proteins from E. coli. Nature. 1970 Jun 27;226(5252):1214–1214. doi: 10.1038/2261214a0. [DOI] [PubMed] [Google Scholar]
- Moore G., Crichton R. R. Reductive methylation: a method for preparing functionally active radioactive ribosomes. FEBS Lett. 1973 Nov 15;37(1):74–78. doi: 10.1016/0014-5793(73)80429-6. [DOI] [PubMed] [Google Scholar]
- Muto A., Ehresmann C., Fellner P., Zimmermann R. A. RNA-protein interactions in the ribosome. I. Characterization and ribonuclease digestion of 16 S RNA-ribosomal protein complexes. J Mol Biol. 1974 Jun 25;86(2):411–432. doi: 10.1016/0022-2836(74)90028-x. [DOI] [PubMed] [Google Scholar]
- Newton I., Brimacombe R. Two specific ribonucleoprotein fragments from the 50-S sub-particle of Escherichia coli ribosomes. Eur J Biochem. 1974 Oct 2;48(2):513–518. doi: 10.1111/j.1432-1033.1974.tb03792.x. [DOI] [PubMed] [Google Scholar]
- Reinbolt J., Schiltz E. The primary structure of ribosomal protein S4 from Escherichia coli. FEBS Lett. 1973 Nov 1;36(3):250–252. doi: 10.1016/0014-5793(73)80383-7. [DOI] [PubMed] [Google Scholar]
- Rice R. H., Means G. E. Radioactive labeling of proteins in vitro. J Biol Chem. 1971 Feb 10;246(3):831–832. [PubMed] [Google Scholar]
- Schulte C., Morrison C. A., Garrett R. A. Protein-ribonucleic acid interactions in Escherichia coli ribosomes. Solution studies on S4-16S ribonucleic acid and L24-23S ribonucleic acid binding. Biochemistry. 1974 Feb 26;13(5):1032–1037. doi: 10.1021/bi00702a031. [DOI] [PubMed] [Google Scholar]
- Ungewickell E., Garrett R., Ehresmann C., Stiegler P., Fellner P. An investigation of the 16-S RNA binding sites of ribosomal proteins S4, S8, S15, and S20 FROM Escherichia coli. Eur J Biochem. 1975 Feb 3;51(1):165–180. doi: 10.1111/j.1432-1033.1975.tb03917.x. [DOI] [PubMed] [Google Scholar]
- Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]
- Zimmermann R. A., Mackie G. A., Muto A., Garrett R. A., Ungewickell E., Ehresmann C., Stiegler P., Ebel J. P., Fellner P. Location and characteristics of ribosomal protein binding sites in the 16S RNA of Escherichia coli. Nucleic Acids Res. 1975 Feb;2(2):279–302. doi: 10.1093/nar/2.2.279. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zimmermann R. A., Muto A., Fellner P., Ehresmann C., Branlant C. Location of ribosomal protein binding sites on 16S ribosomal RNA. Proc Natl Acad Sci U S A. 1972 May;69(5):1282–1286. doi: 10.1073/pnas.69.5.1282. [DOI] [PMC free article] [PubMed] [Google Scholar]