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. 1975 Jun;2(6):943–950. doi: 10.1093/nar/2.6.943

Peptidyl - tRNA hydrolase and RNase activities in cell fractions of rat liver used in in vitro reconstitution of rough membrane.

A A Hochberg, H H Czosnek, E Ziv
PMCID: PMC343480  PMID: 1144067

Abstract

Peptidyl-tRNA hydrolase and RNase activities have been studied in those fractions of rat liver, which are used in in vitro reconstitution of rough membrane, because these enzymes may interfere with the in vitro reconstitution. It was found that smooth membrane has an active peptidyl-tRNA hydrolase, while the other fractions tested, polyribosomes, rough membrane, stripped rough membrane and the post-microsomal supernatant had no, or very low, peptidyl-tRNA hydrolase activity. Polyribosomes, rough and stripped rough membrane have RNase activity; this activity could be completely inhibited by rat liver RNase inhibitor. It is shown that RNase inhibitor is an obligatory component in in vitro experiments, in which rough membrane is reconstituted from stripped rough membrane, ribosomes and mRNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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