Abstract
Mitochondrial DNA from wild-type Saccharomyces cerevisiae and from an "extreme" petite mutant were analyzed by hybridization of several tRNAs on DNA fragments of different buoyant density, obtained by sonication and fractionation on a CsCl gradient. The hybridization patterns show that the genes for tRNAser, tRNAphe, tRNAhis, tRNAval, tRNAileu are present on wild-type mitochondrial DNA, while only genes for tRNAser and tRNAhis are present on petite mitochondrial DNA; moreover the hybridization patterns indicate that these genes are not clustered and suggest that more than one gene might exist for tRNAser and tRNAhis.
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Selected References
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- Aloni Y., Attardi G. Expression of the mitochondria genome in HeLa cells. IV. Titration of mitochondrial genes for 16 s, 12 s and 4 s RNA. J Mol Biol. 1971 Jan 28;55(2):271–276. doi: 10.1016/0022-2836(71)90197-5. [DOI] [PubMed] [Google Scholar]
- Bernardi G., Carnevali F., Nicolaieff A., Piperno G., Tecce G. Separation and characterization of a satellite DNA from a yeast cytoplasmic "petite" mutant. J Mol Biol. 1968 Nov 14;37(3):493–505. doi: 10.1016/0022-2836(68)90117-4. [DOI] [PubMed] [Google Scholar]
- Carnevali F., Falcone C., Frontali L., Leoni L., Macino G., Palleschi C. Informational content of mitochondrial DNA from a "low density" petite mutant of yeast. Biochem Biophys Res Commun. 1973 Apr 2;51(3):651–658. doi: 10.1016/0006-291x(73)91364-8. [DOI] [PubMed] [Google Scholar]
- Carnevali F., Leoni L. Intramolecular heterogeneity of yeast mitochondrial DNA. Biochem Biophys Res Commun. 1972 Jun 28;47(6):1322–1331. doi: 10.1016/0006-291x(72)90217-3. [DOI] [PubMed] [Google Scholar]
- Casey J. W., Hsu H. J., Rabinowitz M., Getz G. S., Fukuhara H. Transfer RNA genes in the mitochondrial DNA of cytoplasmic petite mutants of Saccharomyces cerevisiae. J Mol Biol. 1974 Oct 5;88(4):717–733. doi: 10.1016/0022-2836(74)90395-7. [DOI] [PubMed] [Google Scholar]
- Casey J., Cohen M., Rabinowitz M., Fukuhara H., Getz G. S. Hybridization of mitochondrial transfer RNA's with mitochondrial and nuclear DNA of grande (wild type) yeast. J Mol Biol. 1972 Feb 14;63(3):431–440. doi: 10.1016/0022-2836(72)90438-x. [DOI] [PubMed] [Google Scholar]
- Christiansen C., Christiansen G., Bak A. L. Heterogeneity of mitochondrial DNA from Saccharomyces carlsbergensis: renaturation and sedimentation studies. J Mol Biol. 1974 Mar 25;84(1):65–82. doi: 10.1016/0022-2836(74)90212-5. [DOI] [PubMed] [Google Scholar]
- Cohen M., Casey J., Rabinowitz M., Getz G. S. Hybridization of mitochondrial transfer RNA and mitochondrial DNA in petite mutants of yeast. J Mol Biol. 1972 Feb 14;63(3):441–451. doi: 10.1016/0022-2836(72)90439-1. [DOI] [PubMed] [Google Scholar]
- Cohen M., Rabinowitz M. Analysis of grande and petite yeast mitochondrial DNA by tRNA hybridization. Biochim Biophys Acta. 1972 Oct 11;281(2):192–201. doi: 10.1016/0005-2787(72)90171-2. [DOI] [PubMed] [Google Scholar]
- DUELL E. A., INOUE S., UTTER M. F. ISOLATION AND PROPERTIES OF INTACT MITOCHONDRIA FROM SPHEROPLASTS OF YEAST. J Bacteriol. 1964 Dec;88:1762–1773. doi: 10.1128/jb.88.6.1762-1773.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dawid I. B. Mitochondrial RNA In Xenopus laevis. I. The expression of the mitochondrial genome. J Mol Biol. 1972 Jan 28;63(2):201–216. doi: 10.1016/0022-2836(72)90370-1. [DOI] [PubMed] [Google Scholar]
- HOLLEY R. W. Large-scale preparation of yeast "soluble" ribonucleic acid. Biochem Biophys Res Commun. 1963 Jan 31;10:186–188. doi: 10.1016/0006-291x(63)90048-2. [DOI] [PubMed] [Google Scholar]
- Nass M. M., Buck C. A. Studies on mitochondrial tRNA from animal cells. II. Hybridization of aminoacyl-tRNA from rat liver mitochondria with heavy and light complementary strands of mitochondrial DNA. J Mol Biol. 1970 Dec 14;54(2):187–198. doi: 10.1016/0022-2836(70)90426-2. [DOI] [PubMed] [Google Scholar]
- Prunell A., Bernardi G. The mitochondrial genome of wild-type yeast cells. IV. Genes and spacers. J Mol Biol. 1974 Jul 15;86(4):825–841. doi: 10.1016/0022-2836(74)90356-8. [DOI] [PubMed] [Google Scholar]
