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. 1975 Oct;2(10):1889–1902. doi: 10.1093/nar/2.10.1889

Replication of double-stranded RNA in particles of Penicillium stoloniferum virus S.

K W Buck
PMCID: PMC343554  PMID: 1187348

Abstract

RNA polymerase activity was assayed in different particle classes of Penicillium stoloniferum virus S. RNA polymerase activity was found to be associated with H particles, which contain double-stranded RNA and single-stranded RNA, but not with L particles, which contain only double-stranded RNA and not with M particles, which contain only single-stranded RNA. In H particles the reaction occurred with the formation of one new molecule of double-stranded RNA (or two complementary single strands of RNA) per virus particle and the production of product particles (P particles), which contained two molecules of double-stranded RNA (or its equivalent). This RNA polymerase is therefore a replicase, which catalyses the synthesis of the two complementary strands of double-stranded RNA in a single virus particle. This is the first report of this type of RNA polymerase system.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bozarth R. F., Wood H. A., Mandelbrot A. The Penicillium stoloniferum virus complex: two similar double-stranded RNA virus-like particles in a single cell. Virology. 1971 Aug;45(2):516–523. doi: 10.1016/0042-6822(71)90352-7. [DOI] [PubMed] [Google Scholar]
  2. Buck K. W., Kempson-Jones G. F. Biophysical properties of Penicillium stoloniferum virus S. J Gen Virol. 1973 Mar;18(3):223–235. doi: 10.1099/0022-1317-18-3-223. [DOI] [PubMed] [Google Scholar]
  3. Buck K. W., Ratti G. A model for the replication of double-stranded ribonucleic acid mycoviruses. Biochem Soc Trans. 1975;3(4):542–544. doi: 10.1042/bst0030542. [DOI] [PubMed] [Google Scholar]
  4. Buck K. W., Ratti G. Biophysical and biochemical properties of two viruses isolated from Aspergillus foetidus. J Gen Virol. 1975 May;27(2):211–224. doi: 10.1099/0022-1317-27-2-211. [DOI] [PubMed] [Google Scholar]
  5. Chater K. F., Morgan D. H. Ribonucleic acid synthesis by isolated viruses of Penicillium stoloniferum. J Gen Virol. 1974 Aug;24(2):307–317. doi: 10.1099/0022-1317-24-2-307. [DOI] [PubMed] [Google Scholar]
  6. Faust M., Millward S. In vitro methylation of nascent reovirus mRNA by a virion-associated methyl transferase. Nucleic Acids Res. 1974 Dec;1(12):1739–1752. doi: 10.1093/nar/1.12.1739. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Furuichi Y. "Methylation-coupled" transcription by virus-associated transcriptase of cytoplasmic polyhedrosis virus containing double-stranded RNA. Nucleic Acids Res. 1974 Jun;1(6):809–822. doi: 10.1093/nar/1.6.809. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Furuichi Y., Miura K. A blocked structure at the 5' terminus of mRNA from cytoplasmic polyhedrosis virus. Nature. 1975 Jan 31;253(5490):374–375. doi: 10.1038/253374a0. [DOI] [PubMed] [Google Scholar]
  9. Lapierre H., Astier-Manifacier S., Cornuet P. Activité RNA polymérase associée aux préparations purifiées de virus du Penicillium stoloniferum. C R Acad Sci Hebd Seances Acad Sci D. 1971 Sep 13;273(11):992–994. [PubMed] [Google Scholar]
  10. Lemke P. A., Nash C. H., Pieper S. W. Lytic plaque formation and variation in virus titre among strains of Penicillium chrysogenum. J Gen Microbiol. 1973 Jun;76(2):265–275. doi: 10.1099/00221287-76-2-265. [DOI] [PubMed] [Google Scholar]
  11. Miura K., Watanabe K., Sugiura M., Shatkin A. J. The 5'-terminal nucleotide sequences of the double-stranded RNA of human reovirus. Proc Natl Acad Sci U S A. 1974 Oct;71(10):3979–3983. doi: 10.1073/pnas.71.10.3979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nash C. H., Douthart R. J., Ellis L. F., Van Frank R. M., Burnett J. P., Lemke P. A. On the mycophage of Penicillium chrysogenum. Can J Microbiol. 1973 Jan;19(1):97–103. doi: 10.1139/m73-014. [DOI] [PubMed] [Google Scholar]
  13. Wood H. A. Viruses with double-stranded RNA genomes. J Gen Virol. 1973 Jun;20(Suppl):61–85. doi: 10.1099/0022-1317-20-Supplement-61. [DOI] [PubMed] [Google Scholar]
  14. Zweerink H. J. Multiple forms of SS leads to DS RNA polymerase activity in reovirus-infected cells. Nature. 1974 Feb 1;247(5439):313–315. doi: 10.1038/247313a0. [DOI] [PubMed] [Google Scholar]

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